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Protein Purification Reagents and Kits

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Promotions Available

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's.

Particle Size 65 μm
Shipping Condition Wet Ice
Ligand Type KappaXP affinity ligand
Flow Rate 150 to 300 cm/hr. (Up to 2 bar)
Stationary Phase KappaXP
Type Affinity Matrix
Content And Storage • Operating Temperature: 2°C to 25°C
• Short-term Storage: 2°C to 25°C, avoid direct exposure to sunlight
• Long-term Storage: 2°C to 8°C, protected from light
Matrix Agarose-based
Product Line CaptureSelect
2
Promotions Available

Thermo Scientific™ CaptureSelect™ LambdaXP GoPure™ Column

CaptureSelect LambdaXP GoPure columns are ready-to-use columns designed for fast and easy purification of lambda monoclonal antibodies on standard chromatography systems with high purity and yield in a single step.

Particle Size 65 ±10 μm
Type Pre-packed Column
Content And Storage Store at 2°C to 8°C in dark/protect from light.
Matrix LambdaXP Affinity Matrix
Form Agarose Bead
Max. Operating Pressure 0.2 MPa
Bed Height 25 mm
Product Line CaptureSelect
3
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Product Line HisPur
4

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
5
Promotions Available

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

EDGE
6
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
7

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate consists of a 14 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses.

EDGE
Ligand Type Antibody
Type Conjugate
Target IgG-CH1
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
8
Promotions Available

Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

EDGE
Type Agarose
Stationary Phase Ni-NTA
Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Column Type Affinity Column
9
Promotions Available

Thermo Scientific™ SulfoLink™ Coupling Resin

Covalently immobilize cysteine-peptides or proteins (sulfhydryl-ligands) for affinity purification using iodoacetyl-activated agarose beads and columns.

EDGE
Stationary Phase Haloacetyl
Type Resin
Content And Storage Upon receipt store product in the dark at 4°C. Product is shipped at ambient temperature.
Format Bottle
Column Type Affinity, Agarose Resin
Sufficient For 1 mg Peptide or 10 mg Antibody per mL of Resin
Product Line SulfoLink
10
Promotions Available

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal
11
Promotions Available

Thermo Scientific™ POROS™ CaptureSelect™ IgM-XL Affinity Matrix

Thermo Scientific™ POROS™ CaptureSelect™ IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.

EDGE
Ligand Type CaptureSelect IgM-XL Affinity Ligand
Type Affinity Matrix
Stationary Phase 20% Ethanol
Matrix Cross-linked poly(styrene-divinylbenzene) POROS beads
Binding Capacity >5 g/L
Form Resin Slurry
Product Line POROS CaptureSelect
12

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
13

Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid - proline - glutamic acid - alanine).

EDGE
Ligand Type Antibody
Type Conjugate
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
14
Promotions Available

Thermo Scientific™ CaptureSelect™ C-tagXL GoPure™ Column

CaptureSelect C-tagXL GoPure columns are ready-to-use columns designed for fast and easy purification of C-terminal tagged proteins on standard chromatography systems with high purity and yield in a single step.

EDGE
Particle Size 65 ±10 μm
Type Pre-packed Column
Content And Storage Store at 2°C to 8°C in dark/protect from light.
Matrix C-tagXL Affinity Matrix
Form Agarose Bead
Max. Operating Pressure 0.2 MPa
Bed Height 25 mm
Product Line CaptureSelect
15

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose