Protein Purification Reagents and Kits
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Filtered Search Results
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ Pierce™ Glutathione Agarose
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
| Stationary Phase | Glutathione |
|---|---|
| Type | Agarose |
| Content And Storage | Upon receipt store at 4°C. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Capacity (Metric) | 40 mg Purified Recombinant GST Protein Binding per mL Resin |
| Product Line | Pierce |
Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA
Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA Kit is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect CH1-XL Affinity Matrix.
| Shipping Condition | Dry Ice |
|---|---|
| Type | ELISA Kit |
| Content And Storage | Store in freezer (-5°C to -30°C). |
| Format | Kit |
| Product Line | CaptureSelect |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin
Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.
| Binding Property | Chelator Stable |
|---|
Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix
Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix.
| Particle Size | 65 μm |
|---|---|
| Ligand Type | CH1-XL affinity ligand |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Binding Capacity | >15 mg/mL |
| Form | Agarose Bead |
Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
| Stationary Phase | Divalent Nickel, Metal Chelate |
|---|---|
| Type | Chromatography Cartridge |
| Target | His-Tagged Protein |
| Content And Storage | Store between 4°C and 8°C. Do not freeze. |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |
Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid - proline - glutamic acid - alanine).
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
| Buffer | Lysis Buffer |
|---|---|
| Shipping Condition | Ambient |
| Form | Liquid |
| Product Type | Nuclear and Cytoplasmic Extraction Reagent |
| Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
| Product Line | NE-PER |
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of IgG from multiple species.
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Target | IgG (Fc region) |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate consists of a 14 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses.
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Target | IgG-CH1 |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
| Stationary Phase | Proprietary Ligand |
|---|---|
| Column Type | Proprietary Resin, Affinity |
| Purification Target | Detergent Removal |
Cytiva Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.