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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
3

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

EDGE
4

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
5
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Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur
6

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
7
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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
8

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
9

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
10
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Stationary Phase Divalent Nickel, Metal Chelate
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Product Line HisPur
11

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
12

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
Particle Size 50 to 150 μm
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Type Bulk Resin
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format Bottle
Description 4% agarose, crosslinked
Final Product Type Affinity chromatography equipment
Protein Form Tetrameric protein
Form Resin Slurry
Binding Capacity 20 mg/mL resin
Column Type Suspension
Concentration 50% suspension
13

IBA Lifesciences Strep-Tactin™ 4Flow™ Cartridge

Strep-Tactin™ 4Flow™ cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
Particle Size 50 to 150 μm
Type High Capacity Chromatography Cartridge
Target Twin-Strep-Tag™ and Strep-tag™II
Format Pre-packed Cartridge
For Use With (Application) HPLC/FPLC
Support Type 4% agarose, crosslinked
14
Promotions Available

Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Stationary Phase Metal Chelate, Divalent Cobalt
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Product Line HisPur
15
Promotions Available

Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL

Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.

Stationary Phase Aldehyde
Type Immobilization Kit
Target Antibody, Proteins through Primary Amine
Format Spin Column
Column Type Affinity, Agarose Resin
Capacity (Metric) 2 to 40 mg of protein Immobilization per Column
Sufficient For 2 to 40 mg of Protein per Column
Product Line AminoLink