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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1
Promotions Available

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix

Thermo Scientific™ CaptureSelect™ KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's.

Particle Size 65 ±10 μm
Shipping Condition Wet Ice
Ligand Type KappaXP affinity ligand
Flow Rate 150 to 300 cm/hr. (Up to 2 bar)
Stationary Phase KappaXP
Type Affinity Matrix
Content And Storage Store in refrigerator (2°C to 8°C).
Matrix Agarose-based
Product Line CaptureSelect
2
Promotions Available

Thermo Scientific™ CaptureSelect™ LambdaXP GoPure™ Column

CaptureSelect LambdaXP GoPure columns are ready-to-use columns designed for fast and easy purification of lambda monoclonal antibodies on standard chromatography systems with high purity and yield in a single step.

Particle Size 65 ±10 μm
Type Pre-packed Column
Content And Storage Store at 2°C to 8°C in dark/protect from light.
Matrix LambdaXP Affinity Matrix
Form Agarose Bead
Max. Operating Pressure 0.2 MPa
Bed Height 25 mm
Product Line CaptureSelect
3

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
4
Promotions Available

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

EDGE
5
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
Matrix Magnetic Agarose
6

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate consists of a 14 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses.

EDGE
Ligand Type Antibody
Type Conjugate
Target IgG-CH1
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
7

Thermo Scientific™ POROS™ XS Strong Cation Exchange Resin

Thermo Scientific™ POROS™ XS resin is the first high-capacity, high-resolution resin that allows loading to more than 100 mg/mL capacity in the presence of up to 15 mS/cm NaCl, while delivering unprecedented separation capability.

EDGE
Shipping Condition Ambient
Particle Size 50 μm
Content And Storage Store at 2°C to 30°C. Do not freeze.
Product Line POROS
8
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

EDGE
Shipping Condition Wet Ice
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Product Type Affinity Resin
9
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
10

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
11

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgM Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgM Conjugate consists of a 14 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the μ chain of human, rat, and mouse IgM antibodies.

EDGE
Ligand Type Antibody
Type Conjugate
Target IgM
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
12

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Particle Size 45 to 165 μm
Type Pre-packed Column
Content And Storage 4°C to 30°C
pH Range 3 to 12
Material Polypropylene
Support Type 6% Cross-linked Agarose
13

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Human) Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Hu) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the Fc part (CH3 domain) of all four human IgG subclasses.

EDGE
Ligand Type Antibody
Type Conjugate
Target IgG (Fc region)
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
14

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgA Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgA Conjugate consists of a 14 kDa recombinant single domain antibody fragment that specifically binds to the α-chain of all human IgA antibodies (Fc domain).

EDGE
Ligand Type Antibody
Type Conjugate
Target IgA
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
15
Promotions Available

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

EDGE
Stationary Phase Proprietary Ligand
Column Type Proprietary Resin, Affinity
Purification Target Detergent Removal