Cell Culture Media, Supplements, and Reagents
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Gibco™ RPMI 1640 Medium, HEPES, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Form | Liquid |
|---|---|
| Without Additives | No Glutamine,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ Penicillin-Streptomycin (10,000 U/mL)
For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria
| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | Antibiotic |
| Form | Liquid |
| Shelf Life | 12 Months |
| Concentration | 100 X |
| For Use With (Application) | Prevention of Cell Culture Contamination |
| Sterility | Sterile-filtered |
Gibco™ Trypsin-EDTA (0.05%), phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.
| Chelators | EDTA |
|---|---|
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Classification | Animal Origin |
| Shelf Life | 24 Months |
| Osmolality | 270 - 310 mOsm/kg |
| Sterility | Sterile-filtered |
| Source | Animal Origin |
| Product Line | Gibco |
| Cell Type | Mammalian Cells |
Gibco™ Neurobasal™ Medium
Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement.
Gibco™ Trypsin-EDTA (0.25%), phenol red
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
| Chelators | EDTA |
|---|---|
| Content And Storage | Storage conditions: -5°C to -20°C Shipping conditions: Wet ice Shelf life: 24 months from date of manufacture |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Osmolality | 270 - 320 mOsm/kg |
| Sterility | Sterile-filtered |
| Source | Animal Origin |
| Cell Type | Mammalian Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin |
| Shelf Life | 24 Months |
| Product Line | Gibco |
Gibco™ TrypLE™ Express Enzyme (1X), phenol red
Animal origin-free, recombinant enzyme
| Shipping Condition | Room Temperature |
|---|---|
| Chelators | EDTA |
| Form | Liquid |
| Tested For | In vitro Bioassay |
| Concentration | 1 X |
| Osmolality | Check and Record |
| Sterility | Sterile-filtered |
| Source | Animal origin-free |
| Cell Type | Mammalian Cells |
| Remarks | Room Temperature Stable |
| With Additives | Phenol Red,EDTA |
| Product Type | Cell Culture Dissociation Reagent |
| Classification | Animal Origin-free |
| Culture Type | Adherent Cell Culture |
| Shelf Life | 24 Months |
| Product Line | Gibco |
| Content And Storage | 2 to 8°C |
|---|---|
| Product Type | Sodium Pyruvate |
| Form | Liquid |
| Shelf Life | 12 Months |
| Certifications/Compliance | FDA, ISO 13485 |
| For Use With (Application) | Mammalian Cell Culture |
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
Gibco™ MEM Non-Essential Amino Acids are used as a supplement for cell culture medium, to increase cell growth and viability.
| Content And Storage | Store at 2–8°C. Protect from light. |
|---|---|
| Product Type | Amino Acid Supplement |
| Form | Liquid |
| pH | 0.9 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 18 Months |
| Concentration | 100X |
| Sterility | Sterile-filtered |
| Cell Type | Mammalian Cells |
Gibco™ RPMI 1640 Medium
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
| Content And Storage | Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|---|---|
| Culture Environment | CO2 |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1X |
| Osmolality | 260 - 310 mOsm/kg |
| Sterility | Sterile-filtered |
| Cell Type | Mammalian Cells |
| With Additives | Glutamine,Phenol Red,Sodium Bicarbonate |
| Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
| Classification | Animal Origin-free |
| pH | 7.0 to 7.4 |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |
Gibco™ DMEM, high glucose, GlutaMAX™ Supplement
DMEM, high glucose, GlutaMAX™ Supplement
| Content And Storage | 2 to 8°C |
|---|---|
| With Additives | High Glucose,GlutaMAX,Phenol Red |
| Product Type | Dulbecco's Modified Eagle Medium |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| pH | 6.8 to 7.2 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
| Shipping Condition | Ambient |
|---|---|
| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
| Content And Storage | Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 18 months from date of manufacture |
| Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1X |
| Sterility | Sterile-filtered |
| Cell Type | Mammalian Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine,Phenol Red |
| Product Type | Opti-MEM I |
| Classification | Human Origin |
| Shelf Life | 18 Months From Date of Manufacture |
| Product Line | Gibco, Opti-MEM |
| Content And Storage | 2 to 8°C |
|---|---|
| Description | DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With GlutaMAX™, Phenol Red, Sodium Pyruvate, 6.8 to 7.2 pH, 12 Months Shelf Life, 290 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
| With Additives | GlutaMAX,Phenol Red,Sodium Pyruvate |
| Product Type | DMEM/F-12 Medium |
| Form | Liquid |
| Without Additives | No HEPES |
| pH | 6.8 to 7.2 |
| Certifications/Compliance | ISO 13485 standards, FDA |
| Shelf Life | 12 Months |
| Concentration | 1 X |
| For Use With (Application) | Mammalian cell culture |
Gibco™ Antibiotic-Antimycotic (100X)
Solution contains penicillin, streptomycin, and Gibco™ Amphotericin B to prevent cell culture contamination.
| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | Antibiotic-Antimycotic |
| Form | Liquid |
| Culture Type | Mammalian Cell Culture |
| Shelf Life | 12 Months |
| Concentration | 100 X |
| Sterility | Sterile-filtered |
Gibco™ McCoy's 5A (Modified) Medium
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.
| Cell Line | Rat fibroblasts |
|---|---|
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Sterilization Method | Sterile-filtered |
| Product Type | McCoy's 5A Modified Medium |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Classification | Animal Origin |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months From Date of Manufacture |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Product Line | Gibco |
| Cell Type | Biopsy Tissues |
Gibco™ DMEM, high glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
|---|