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Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.
DMEM Cell Culture Media (305)
Additional Selective and Differential Cell Culture Media (267)
MEM Cell Culture Media (122)
RPMI Cell Culture Media (100)
DMEM-F-12 Cell Culture Media (50)
Medium199 Cell Culture Media (27)
Ham's F-12 Cell Culture Media (23)
IMDM Cell Culture Media (23)
McCoy's 5A Cell Culture Media (17)
Ham's F-10 Cell Culture Media (16)
Serum-Free Cell Culture Media (13)
Liebovitz's L-15 Cell Culture Media (13)
BME Cell Culture Media (6)
Reduced Serum Cell Culture Media (3)

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1

Gibco™ DMEM, no glucose
GREENER_CHOICE

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
2
Promotions Available

Gibco™ FreeStyle™ F17 Expression Medium
GREENER_CHOICE

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

EDGE
Cell Line HEK293
Culture Environment CO2
Purity or Quality Grade Qualified for Further Manufacturing
Form Liquid
Without Additives No Glutamine
Endotoxin Level < 1 EU/mL
Concentration 1 X
For Use With (Application) Protein expression, viral vector production
Sterility Sterile
Product Type Expression Medium
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Product Line Freestyle
Serum Level Serum-free
3

Gibco™ DMEM/F-12, HEPES, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
4
Promotions Available

Gibco™ MEM, GlutaMAX™ Supplement
GREENER_CHOICE

MEM, GlutaMAX™ Supplement

EDGE
Content And Storage 2 to 8°C
With Additives GlutaMAX,Phenol Red
Product Type Minimum Essential Medium
Form Liquid
Without Additives No HEPES,No Sodium Pyruvate
pH 7.0 to 7.4
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
5

Gibco™ MEM α, nucleosides
GREENER_CHOICE

MEM α, nucleosides

EDGE
Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description MEM α± Medium, 500mL, 1 X Concentration, Liquid Form, With Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red, Sodium Pyruvate, 7 to 7.4 pH, 12 Months Shelf Life, 280 to 310mOsm/kg Osmolality, Used for Mammalian Cell Culture
With Additives Glutamine,Phenol Red,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type MEM α± Medium
Form Liquid
pH 7 to 7.4
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
6
Promotions Available

Gibco™ MEM, GlutaMAX™ Supplement
GREENER_CHOICE

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

EDGE
Shipping Condition Room Temperature
Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No HEPES,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Rat Astrocytes
Sterilization Method Sterile-filtered
With Additives Low Glucose,GlutaMAX,Phenol Red
Product Type MEM (Minimum Essential Medium)
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco, GlutaMAX
Serum Level Standard Serum Supplementation
7

Gibco™ RPMI 1640 Medium
GREENER_CHOICE

RPMI 1640 Medium

EDGE
Regulatory Status For in-vitro diagnostic use
Product Type RPMI 1640 Medium
Form Liquid
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
8

Gibco™ Advanced DMEM
GREENER_CHOICE

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

EDGE
Shipping Condition Room Temperature
Cell Line MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Reduced Serum
9
Promotions Available

Gibco™ IMDM, GlutaMAX™ Supplement
GREENER_CHOICE

IMDM, GlutaMAX™ Supplement

EDGE
Content And Storage 2 to 8°C
With Additives GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type Iscove's modified Dulbecco's medium
Form Liquid
Without Additives No α±-Thioglycerol,No 2-Mercaptoethanol
pH 6.9 to 7.3
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
10
Promotions Available

Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement
GREENER_CHOICE

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

EDGE
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
11

Gibco™ Opti-MEM™ I Reduced Serum Medium
GREENER_CHOICE

Opti-MEM™ I Reduced Serum Medium

EDGE
Content And Storage 2 to 8°C
With Additives Glutamine,Phenol Red,Sodium Pyruvate
Product Type Reduced-Serum Medium
Form Liquid
pH 7.0 to 7.4
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 18 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture, transfection
12

Keratinocyte-SFM Medium (Kit) with L-glutamine, EGF, and BPE
GREENER_CHOICE

EDGE
13

Gibco™ MEM, no glutamine
GREENER_CHOICE

MEM, no glutamine

EDGE
Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description MEM Medium, 500mL, 1 X Concentration, Liquid Form, With Phenol Red, 7 to 7.4 pH, 12 Months Shelf Life, 280 to 320mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Soluble in Water, Used for Mammalian Cell Culture
With Additives Phenol Red
Product Type MEM Medium
Form Liquid
Without Additives No Glutamine,No HEPES
pH 7 to 7.4
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
14

Gibco™ DMEM, high glucose, no glutamine
GREENER_CHOICE

DMEM, high glucose, no glutamine

EDGE
15
Promotions Available

Gibco™ Ham's F-12 Nutrient Mix
GREENER_CHOICE

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

EDGE
Cell Line CHO, COS-7, and rat prostate epithelial cells
Sterilization Method Sterile-filtered
With Additives Glutamine,Phenol Red,Sodium Pyruvate
Product Type Ham's F-12 Nutrient Mixture
Form Liquid
Without Additives No HEPES
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months From Date of Manufacture
Concentration 1 X
Sterility Sterile-filtered
Product Line Gibco
Cell Type Primary Rat Astrocytes