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Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.
DMEM Cell Culture Media (305)
Additional Selective and Differential Cell Culture Media (267)
MEM Cell Culture Media (122)
RPMI Cell Culture Media (100)
DMEM-F-12 Cell Culture Media (50)
Medium199 Cell Culture Media (26)
Ham's F-12 Cell Culture Media (23)
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McCoy's 5A Cell Culture Media (17)
Ham's F-10 Cell Culture Media (16)
Serum-Free Cell Culture Media (13)
Liebovitz's L-15 Cell Culture Media (13)
BME Cell Culture Media (6)
Reduced Serum Cell Culture Media (3)

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1
Promotions Available

Gibco™ Opti-MEM™ I Reduced Serum Medium
GREENER_CHOICE

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

EDGE
Shipping Condition Room Temperature
Cell Line Sp2, AE-1, CHO, BHK-21, and HEK
Content And Storage Store at 2–8°C and protect from light.
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Fibroblasts
Sterilization Method Sterile-filtered
With Additives Glutamine,Phenol Red
Product Type Opti-MEM I
Classification Human Origin
Shelf Life 18 Months From Date of Manufacture
Product Line Gibco, Opti-MEM
2
Promotions Available

Gibco™ FreeStyle™ F17 Expression Medium
GREENER_CHOICE

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

EDGE
Cell Line HEK293
Culture Environment CO2
Purity or Quality Grade Qualified for Further Manufacturing
Form Liquid
Without Additives No Glutamine
Endotoxin Level < 1 EU/mL
Concentration 1 X
For Use With (Application) Protein expression, viral vector production
Sterility Sterile
Product Type Expression Medium
Classification Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type Suspension Cell Culture
Product Line Freestyle
Serum Level Serum-free
3
Promotions Available

Gibco™ DMEM, low glucose, GlutaMAX™ Supplement, pyruvate
GREENER_CHOICE

DMEM, low glucose, GlutaMAX™ Supplement, pyruvate

EDGE
Regulatory Status For in-vitro diagnostic use
Content And Storage Store at 2–8°C.
Description DMEM Medium, 500mL, 1 X Concentration, Liquid Form, With Low Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red, 7 to 7.2 pH, 12 Months Shelf Life, 300 to 340mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives Low Glucose,Sodium Pyruvate,GlutaMAX,Phenol Red
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Form Liquid
Without Additives No HEPES
pH 7 to 7.2
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1X
For Use With (Application) Mammalian cell culture
4

Keratinocyte-SFM Medium (Kit) with L-glutamine, EGF, and BPE
GREENER_CHOICE

EDGE
5
Promotions Available

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, HEPES
GREENER_CHOICE

DMEM, high glucose, GlutaMAX™ Supplement, HEPES

EDGE
Content And Storage 2 to 8°C
With Additives High Glucose,GlutaMAX,Phenol Red,HEPES,Phenol red
Product Type Dulbecco's Modified Eagle Medium
Form Liquid
Without Additives No Sodium Pyruvate
pH 7.1 to 7.3
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 12 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture
6
Promotions Available

Gibco™ DMEM, high glucose, no glutamine, no methionine, no cystine
GREENER_CHOICE

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
7
Promotions Available

Gibco™ Opti-MEM™ I Reduced Serum Medium
GREENER_CHOICE

Opti-MEM™ I Reduced Serum Medium

EDGE
Content And Storage 2 to 8°C
With Additives Glutamine,Phenol Red,Sodium Pyruvate
Product Type Reduced-Serum Medium
Form Liquid
pH 7.0 to 7.4
Certifications/Compliance ISO 13485 standards, FDA
Shelf Life 18 Months
Concentration 1 X
For Use With (Application) Mammalian cell culture, transfection
8
Promotions Available

Gibco™ DMEM/F-12, HEPES, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
9

Gibco™ DMEM, high glucose, no glutamine
GREENER_CHOICE

DMEM, high glucose, no glutamine

EDGE
10

Gibco™ Advanced MEM
GREENER_CHOICE

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

EDGE
Shipping Condition Ambient
Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type MEM (Minimum Essential Medium)
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Reduced Serum
11
Promotions Available

Gibco™ Medium 254
GREENER_CHOICE

Medium 254

EDGE
12
Promotions Available

Gibco™ MEM α, nucleosides, no ascorbic acid
GREENER_CHOICE

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

EDGE
13
Promotions Available

Gibco™ MEM α, nucleosides, no phenol red
GREENER_CHOICE

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

EDGE
Shipping Condition Room Temperature
Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No HEPES,No Phenol Red
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Rat Astrocytes
Sterilization Method Sterile-filtered
With Additives Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type MEM α (Minimum Essential Medium α)
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco
Serum Level Standard Serum Supplementation
14

Gibco™ RPMI 1640 Medium, HEPES, no glutamine
GREENER_CHOICE

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

EDGE
Form Liquid
Without Additives No Glutamine,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months
Concentration 1 X
15

Gibco™ BenchStable™ DMEM
GREENER_CHOICE

Gibco DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Shipping Condition Room Temperature
Cell Line HeLa, 293, Cos-7, and PC-12
Content And Storage Store at room temperature. Protect from light.
Form Liquid
Without Additives No Sodium Pyruvate,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 9001: 2015 standard
Green Features ACT Ecolabel (Silver)
Concentration 1 X
Sterility Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line BenchStable
Serum Level Standard Serum Supplementation