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Drug Development (4)
Genome Editing (2)
Scarless Cloning (62)
Signal Transduction (1)
Traditional Cloning (459)

Sensitive to Heat Inactivation

No (68)
Yes (437)

Optimal Reaction Temperature

30°C (9)
37°C (444)
50°C (2)
55°C (21)
65°C (25)

Methylation Sensitivity

CpG Methylation-Sensitive (239)
Dam Methylation-Sensitive (37)
Dcm Methylation-Sensitive (22)
Not CpG Methylation-Sensitive (288)
Not Dam Methylation-Sensitive (457)
Not Dcm Methylation-Sensitive (444)

1 – 15 of 1,599 results

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

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Pricing and Availability
Specifications

Reverse Transcriptases

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 10⁹ cfu/μg plasmid DNA.

Promotions are available

Pricing and Availability
Specifications

Competent Cells

Shipping Condition	Dry Ice
Blue/White Screening	Yes (lacZΔM15)
Cloning Methylated DNA	Yes (mcrA)
Transformation Efficiency Level	High Efficiency (>1 x 10⁹ cfu/μg)
High-throughput Compatibility	Low
Improves Plasmid Quality	Yes (endA1)
Preparing Unmethylated DNA	No
Plasmid	High Copy Plasmid
Reduces Recombination	Yes (recA1)
T1 Phage - Resistant (tonA)	No
Antibiotic Resistance Bacterial	Yes (Streptomycin)
Format	Tube
Product Type	Chemically Competent Cells
Species	E. coli (K12)
Product Line	One Shot™
Contains F' Episome	No

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Pricing and Availability
Specifications

Direct PCR Reagents and Kits

Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Invitrogen™ dNTP Set (100 mM)

Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.

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Pricing and Availability
Specifications

dNTPs

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	100 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Promotions are available

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	EcoR I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

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Pricing and Availability
Specifications

Modifying Enzymes

Product Type	T4 DNA Ligase
Enzyme	DNA Ligase
Compatible Buffer	10X T4 DNA Ligase Buffer
Concentration	5 U/μL

Thermo Scientific™ FastDigest XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Promotions are available

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Xba I
Methylation Sensitivity	Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

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Pricing and Availability
Specifications

cDNA Synthesis Kits and Master Mixes

GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Quantity	Each
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ Dynabeads™ M-280 Streptavidin

Dynabeads M-280 Streptavidin beads are 2.8-μm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin.

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Pricing and Availability
Specifications

DNA Extraction and Purification

Shipping Condition	Ambient Temperature
Surface Functionality	Tosylactivated, Hydrophobic
Color	Brown
Binding Property	650-900 pmoles/mg beads
Concentration	10 mg/mL
High-throughput Compatibility	High-throughput Compatible
For Use With (Application)	Capture of Biotinylated Targets
Ligand Type	Streptavidin
Regulatory Status	For Research Use Only
Format	Beads in suspension
Bead Type	Magnetic Polystyrene
Product Type	Streptavidin Magnetic Beads
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Sample Type	Any Sample Type
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Shelf Life	36 months from date of manufacture
Uniformity	Monosized 2.8 μm (CV <5%)
Product Line	Dynabeads™

Invitrogen™ UltraPure™ Salmon Sperm DNA Solution

Used directly in preparation of prehybridization and hybridization solutions

Promotions are available

Pricing and Availability

DNA Extraction and Purification

Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix

Catalyzes in vitro recombination of PCR products to generate entry clones

Promotions are available

Pricing and Availability
Specifications

Modifying Enzymes

Shipping Condition	Dry Ice
Content And Storage	Gateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 μg/μl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type	BP Clonase Enzyme Mix
Enzyme	BP Clonase
Compatible Buffer	Enzyme Buffer
Product Line	Clonase™, Gateway™

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Pricing and Availability
Specifications

Standard PCR Reagents and Kits

Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	5 U/μL

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing and Availability
Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2X
For Use With (Application)	Standard PCR,High-fidelity PCR

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

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Pricing and Availability

Restriction Enzymes

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Promotions are available

Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BpiI (BbsI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

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