Enzymes and Inhibitors

Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI). XHOI 50U/UL 10000U

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). Restriction enzyme, FastDigest BpiI, 20µL

Thermo Scientific™ FastDigest SanDI (KflI)

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest KflI, 20µL

Thermo Scientific™ FastDigest HinP1I (Hin6I)

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Hin6I, 200µL

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ DraI

Cut at TTT^AAA sites with DraI restriction enzyme, which performs best at 37°C in Tango buffer. RESTRICTION ENZYME, CONVENTIONAL, DRAI, 50U/UL,cuts TTT¬AAA at 37°C (suppliedwith 2mL of 10X

Thermo Scientific™ KspAI (HpaI)

The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI). KSPAI (HPAI) 10U/UL 2500U

Thermo Scientific™ NotI

The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). NOTI 10U/UL 500U

Thermo Scientific™ PsyI (Tth111I)

Cut at GACN^NNGTC sites with PsyI (Tth111I) restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: AspI, PflFI, Tth111I). RESTRICTION ENZYME, CONVENTIONAL, PSYI (TTH111I),10u/ul, cuts GACN¬NNGTC at 37°C (supplied with

Thermo Scientific™ Buffer R

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer R, premixed with BSA for enhanced stability. 10X BUFFER R 5X1 ML

Thermo Scientific™ FastDigest PvuI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST PVUI 20UL

Thermo Scientific™ FastDigest AscI (SgsI)

Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI). Restriction enzyme, FastDigest SgsI, 100µL

Thermo Scientific™ FastDigest HhaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 200 UL RESTRICTION ENZYME, FASTDIGEST, HHAI, CUTSGCG¬C, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest Bsu36I (Eco81I)

Cut at CC^TNAGG sites with Thermo Scientific™ FastDigest™ Bsu36I (Eco81I), which performs best using universal FastDigest Buffer (Isoschizomers: AxyI, Bse21I). Restriction enzyme, FastDigest Eco81I, 50µL

Thermo Scientific™ BsuRI (HaeIII)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). RESTRICTION ENZYME, CONVENTIONAL, BSURI (HAEIII),10u/µL cuts GG¬CC at 37°C (supplied with 1mL of

Thermo Scientific™ FastDigest BsmBI (Esp3I)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Esp3I, 20µL

Thermo Scientific™ FastDigest TaqI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, TAQI, CUTST¬CGA, 1µL cleaves 1µg DNA in 5 minutes at 65°C

Thermo Scientific™ PdmI (XmnI)

The PdmI (XmnI) restriction enzyme recognizes GAANN^NNTTC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Asp700I, MroXI, XmnI). PDMI (XMNI) 10U/UL 2500U

Thermo Scientific™ BseNI (BsrI)

Cut at ACTGG(1/-1)^ sites with BseNI (BsrI) restriction enzyme, which performs best at 65°C in B buffer (Isoschizomers: Bse1I, BsrI, BsrSI). BSENI (BSRI) 10U/UL 5000U

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 10U/UL 5000U

Thermo Scientific™ FastDigest PspFI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, PSPFI, CUTSCCCAGC(-5/-1)¬, 1µL cleaves 1µg DNA in 5 minutes

Thermo Scientific™ FastDigest™ SpeI (BcuI)

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI). Restriction enzyme, FastDigest BcuI, 20µL

Thermo Scientific™ PvuII

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer. RESTRICTION ENZYME, CONVENTIONAL, PVUII, 50U/UL,HC, cuts CAG¬CTG at 37°C (supplied with 1mL of

Thermo Scientific™ BseGI (BtsCI)

Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI). BSEGI (BTSCI) 10U/UL 500U

Thermo Scientific™ FastDigest AcuI (Eco57I)

Cut at CTGAAG(16/14)^ sites with Thermo Scientifc™ FastDigest™ AcuI (Eco57I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Eco57I, 20µL

Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I). RESTRICTION ENZYME, CONVENTIONAL, KPNI, 10U/UL,cuts GGTAC¬C at 37°C (suppliedwith 1mL of 10X

Thermo Scientific™ FastDigest BsiWI (Pfl23II)

Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI). Restriction enzyme, FastDigest Pfl23II, 50µL

Thermo Scientific™ SfaAI (AsiSI)

Cut at GCGAT^CGC sites with SfaAI (AsiSI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsiSI, RgaI, SgfI). 1000 UNIT RESTRICTION ENZYME, CONVENTIONAL, SFAAI(AsiSI), 10u/ul, cuts GCGAT¬CGC at 37°C(supplied

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