Enzymes and Inhibitors

Thermo Scientific™ DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI). DPNI 10U/UL 2500U

Thermo Scientific™ FastDigest™ XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 750 UL RESTRICTION ENZYME, FASTDIGEST, XBAI, CUTST¬CTAGA, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ FastDigest AscI (SgsI)

Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI). Restriction enzyme, FastDigest SgsI, 100µL

Thermo Scientific™ FastDigest BbsI (BpiI)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I). Restriction enzyme, FastDigest BpiI, 20µL

Thermo Scientific™ NcoI

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I). 2500 UNIT RESTRICTION ENZYME, CONVENTIONAL, NCOI,10u/uL, cuts C¬CATGG at 37°C (suppliedwith 1mL of

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. BAMHI 10U/UL 10000U

Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI). XHOI 10U/UL 5000U

Thermo Scientific™ FastDigest™ EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer. X5 FASTDIGEST ECORI 500UL

Thermo Scientific™ EcoRI

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 10U/UL 5X5000U

Thermo Scientific™ BcuI (SpeI)

Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI). RESTRICTION ENZYME, CONVENTIONAL, BCUI (SPEI),10u/ul, cuts A¬CTAGT at 37°C (supplied with 1mL

Thermo Scientific™ FastDigest TaqI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, TAQI, CUTST¬CGA, 1µL cleaves 1µg DNA in 5 minutes at 65°C

Thermo Scientific™ FastDigest™ NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). 250 UL RESTRICTION ENZYME, FASTDIGEST, NOTI, CUTSGC¬GGCCGC, 1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ FastDigest SalI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. FASTDIGEST SALI 200UL

OPTIZYME™ EcoRI, Fisher BioReagents™

Optizyme EcoR I restriction enzyme, high concentration

Thermo Scientific™ FastDigest BglII

Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST BGLII 100UL

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ FastDigest AluI

Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI). 100 UL RESTRICTION ENZYME, FASTDIGEST, ALUI, CUTSAG¬CT, 1µL cleaves 1µg DNA in 15 minutes at 37°C

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system. X5 Buffer set for restriction enzymes includes

Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). PAEI (SPHI) 10U/UL 2500U

Thermo Scientific™ FastDigest HpaII

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 200 UL RESTRICTION ENZYME, FASTDIGEST, HPAII,cuts C¬CGG,1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ FastDigest AclI (Psp1406I)

Cut at AA^CGTT sites with Thermo Scientific™ FastDigest™ AclI (Psp1406I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. Restriction enzyme, FastDigest Psp1406I, 20µL

Thermo Scientific™ MssI (PmeI)

The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI). MSSI (PMEI) 5U/UL 250U

Thermo Scientific™ MluI

The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer. MLUI 10U/UL 1000U

Thermo Scientific™ FastDigest BspMI (BveI)

Cut at ACCTGC(4/8)^ sites with Thermo Scientific™ FastDigest™ BspMI (BveI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc36I, BfuAI). Restriction enzyme, FastDigest BveI, 50µL

Thermo Scientific™ KpnI

The KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Asp718I). RESTRICTION ENZYME, CONVENTIONAL, KPNI, 50U/UL,HC, cuts GGTAC¬C at 37°C (supplied with 4mL of

Thermo Scientific™ Nb.Mva1269I

Nick at GAATGC sites with Nb.Mva1269I nicking enzyme, which performs best at 37°C in O buffer. RESTRICTION ENZYME, NICKING, NB.MVA1269I, 10U/UL,cuts CCTAC¬G (lower strand) at 37°C(supplied with

Thermo Scientific™ Alw44I

Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI). ALW44I 10U/UL 1000U

Thermo Scientific™ NmuCI (Tsp45I)

Cut at ^GTSAC sites with NmuCI (Tsp45I) restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: Tsp45I). RESTRICTION ENZYME, CONVENTIONAL, NMUCI (TSP45I),10u/ul, cuts ¬GTSAC at 37°C (supplied with 1mL of

Thermo Scientific™ Hpy8I (MjaIV)

The Hpy8I (MjaIV) restriction enzyme recognizes GTN^NAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: Hpy166II). RESTRICTION ENZYME, CONVENTIONAL, HPY8I (MJAIV),10u/ul, cuts GTN¬NAC at 37°C (supplied with 1mL

Thermo Scientific™ BclI

Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I). BCLI 10U/UL 5000U

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