Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ Pierce™ Cysteine-HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Invitrogen™ Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester)
Acryloyl-X, SE (6-((acryloyl)amino)hexanoic acid, succinimidyl ester) is an amine-reactive molecule that enables crosslinking of acrylamide to proteins and other biomolecules containing amines.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Invitrogen™ Alexa Fluor™ 647 C2 Maleimide
Alexa Fluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines.
Thermo Scientific™ Pierce™ MMTS (methyl methanethiosulfonate)
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Thermo Scientific™ DyLight™ Antibody Labeling Kits
Covalently label antibodies or proteins with DyLight dyes for use in immunofluorescence, flow cytometry, western blot analysis, and high content analysis.
Invitrogen™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Thermo Scientific™ 1M TEAB and 50% Hydroxylamine Buffers for TMT Experiments
Common TMT buffers such as 1M Triethylammonium bicarbonate (TEAB) and 50% Hydroxylamine for isobaric mass tag labeling experiments.
Invitrogen™ Alexa Fluor™ 488 C5 Maleimide
Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nm laser line.
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.