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  6. Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Product Code. 15597520 Shop All Thermo Scientific Products
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Length (Metric):
5 mm
15 mm
20 mm
50 mm
150 mm
250 mm
500 mm
750 mm
Diameter (Metric):
0.05 mm
0.075 mm
0.1 mm
0.2 mm
0.3 mm
0.75 mm
75 μm
1 mm
Particle Size:
2 μm
3 μm
5 μm
Type:
Analytical Column
Analytical Column, NanoViper
Analytical nano Column
Column
Trap Cartridge
Trap Column
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Each
Pack of 2
Pack of 5
Codice prodotto. Length (Metric) Diameter (Metric) Particle Size Type unitSize
15597520 250 mm 0.75 mm 2 μm Analytical Column Each
11342263 50 mm 0.075 mm 3 μm Analytical Column, NanoViper Each
11352263 150 mm 0.075 mm 3 μm Analytical Column Each
11362263 250 mm 0.075 mm 3 μm Analytical Column Each
11372263 500 mm 0.075 mm 3 μm Analytical Column Each
11302273 150 mm 1 mm 3 μm Analytical Column Each
11312113 5 mm 1 mm 5 μm Trap Cartridge Each
11362113 5 mm 0.3 mm 5 μm Trap Cartridge Pack of 5
11322113 15 mm 1 mm 5 μm Trap Cartridge Each
11382243 150 mm 0.1 mm 5 μm Trap Column Pack of 2
11332253 150 mm 0.2 mm 5 μm Trap Column Pack of 2
11392243 150 mm 0.1 mm 5 μm Trap Column Pack of 2
11352103 150 mm 0.075 mm 5 μm Analytical Column Each
11362103 250 mm 0.075 mm 5 μm Analytical Column Each
11352023 150 mm 0.05 mm 2 μm Analytical Column, NanoViper Each
11372013 150 mm 0.3 mm 2 μm Analytical Column Each
11392253 50 mm 0.75 mm 2 μm Analytical Column, NanoViper Each
11382013 500 mm 0.75 mm 2 μm Analytical Column Each
11342013 150 mm 0.075 mm 2 μm Analytical Column Each
11342023 50 mm 0.05 mm 2 μm Analytical Column, NanoViper Each
11332023 50 mm 0.3 mm 2 μm Analytical Column Each
11352013 150 mm 0.075 mm 3 μm Trap Column Pack of 2
15810705 500 mm 0.075 mm 2 μm Analytical Column Each
15820705 500 mm 0.075 mm 3 μm Analytical Column Each
15567530 500 mm 0.075 mm 2 μm Analytical Column Each
15572920 750 mm 0.075 mm 2 μm Analytical nano Column Each
15800705 50 mm 1 mm 2 μm Analytical Column, NanoViper Each
15588090 20 mm 75 μm 3 μm Trap Column Each
12739835 150 mm 0.05 mm 2 μm Analytical Column Each
15527660 150 mm 0.05 mm 2 μm Analytical Column Each
11382253 250 mm 0.075 mm 3 μm Analytical Column Each
11342103 150 mm 0.075 mm 3 μm Analytical Column Each
11312263 150 mm 0.1 mm 5 μm Trap Column Pack of 2
15517460 150 mm 0.075 mm 2 μm Analytical Column Each
17294434 150 mm 0.1 mm 5 μm Trap Column Pack of 2
17204444 150 mm 0.3 mm 3 μm Analytical Column, NanoViper Each
13486836 150 mm 1 mm 2 μm Analytical Column Each
15518080 250 mm 0.05 mm 2 μm Column Each
15588080 500 mm 0.05 mm 2 μm Column Each
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Codice prodotto. 15597520

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Descrizione

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation.

  • High resolution in protein identification, biomarker discovery and systems biology
  • Particular loadability allows hghest sensitivity in LC/MS
  • Designed for TFA-free LC-MS, minimizing ion-suppression effects
  • Optimized for coupling to ESI/MS and MALDI-MS
  • Highest column-to-column reproducibility
  • Easy-to-use, cutting-edge miniaturized HPLC
  • Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

  • Ease of use, no sleeve for small capillaries
  • Tool-free assembly
  • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third party valves and unions
  • No experimental failure due to bad connections
Ideal for high-resolution peptide mapping of tryptic, natural and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery and systems biology.
  • High loading capcity
  • Use for the analysis of low abundant peptides often found in complex proteomics samples
  • Select the column length and I.D. for your throughput, sensitivity and amount of sample available requirements
  • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D.
  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

PepMap nano columns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one dimensional peptide mapping experiments and 2D-LC analyses.

TRUSTED_SUSTAINABILITY

Specifica

Column Format Analytical Column, NanoViper
Diameter (Metric) 0.75 mm
Flow Rate 300 nL/min
Length (Metric) 250 mm
Max. Pressure 11600 psi (800 bar)
Particle Size 2 μm
pH 2 to 8
Pore Size 100 Å
Surface Area 450 m²/g
Temperature 60°C
USP Type L1
Stationary Phase C18
Packing Material Spherical, Fully Porous, Ultrapure Silica
Carbon Load 15%
Column Type Reversed Phase
Endcapped Yes
Phase Reversed Phase
Material PEEK Shielded Fused Silica
Type Analytical Column
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Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

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