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  6. CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Red 685, eBioscience™, Invitrogen™

CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Product Code. 17276546
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Quantity:
100 Tests
25 Tests
Unit Size:
Pack of 100
Pack of 25
Product Code. Quantity unitSize
17276546 25 Tests Pack of 25
17226566 100 Tests Pack of 100
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Product Code. 17276546

Brand: Invitrogen H007T02R02

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Description

Mouse Monoclonal Antibody

The MEM-15 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This MEM-15 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MEM-15 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 4 μL (0.6 μg) per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.

Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser

CD14 Monoclonal antibody specifically detects CD14 in Human, Non-human Primate samples. It is validated for Flow Cytometry
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD14
Applications Flow Cytometry
Classification Monoclonal
Clone MEM-15
Concentration 4 μL/Test
Conjugate NovaFluor Red 685
Formulation PBS with no preservative; pH 7.2
Gene Cd14
Gene Accession No. P08571
Gene Alias CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Host Species Mouse
Purification Method Affinity chromatography
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 929
Target Species Human, Non-human Primate
Content And Storage 4° C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1
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