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CD56 (NCAM) Monoclonal Antibody (TULY56), PE-Cyanine5, eBioscience™, Invitrogen™

Product Code. 16536920
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Quantity:
100 Tests
25 Tests
Unit Size:
Pack of 100
Pack of 25
Product Code. Quantity unitSize
16536920 25 Tests Pack of 25
16546920 100 Tests Pack of 100
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Product Code. 16536920

Brand: Affymetrix eBioscience 15056641

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Mouse Monoclonal Antibody

Description: This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells. Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB. The TULY56 monoclonal antibody crossreacts with Rhesus macaque. Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TULY56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD56 (NCAM)
Applications Flow Cytometry
Classification Monoclonal
Clone TULY56
Concentration 5 μl/Test
Conjugate PE-Cyanine5
Formulation PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene NCAM1
Gene Accession No. P13591
Gene Alias CD56; MSK39; NCAM; NCAM1
Gene Symbols NCAM1
Host Species Mouse
Purification Method Affinity Chromatography
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 4684, 693789
Target Species Human, Non-human Primate, Rhesus Monkey
Content And Storage 4° C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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