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  5. Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates

Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates
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Product Code. 10547781
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Surface Treatment:
Enhanced Binding
Immulon 1 B
Immulon 2 HB
Immulon 4 HBX
MaxiSorp
MediSorp
MultiSorp
PolySorp
Universal Binding
Certified:
No
Yes
Volume (Metric) Working:
230 μL
280 μL
330 μL
350 μL
Barcode:
Includes Barcode
No Barcode
Unit Size:
Pack of 180
Pack of 50
Pack of 60
13 product options available for selection
Product selection table with 13 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Surface Treatment Certified Volume (Metric) Working Barcode unitSize
10547781 MaxiSorp No 350 μL No Barcode Pack of 60
10547915 Immulon 1 B No 230 μL No Barcode Pack of 50
10795026 Immulon 2 HB No 280 μL No Barcode Pack of 50
10468075 Immulon 4 HBX No 280 μL No Barcode Pack of 50
10394751 MaxiSorp Yes 350 μL No Barcode Pack of 60
10498802 PolySorp No 350 μL No Barcode Pack of 180
10294401 MaxiSorp Yes 350 μL No Barcode Pack of 180
10264451 MaxiSorp Yes 350 μL Includes Barcode Pack of 180
10275411 MediSorp Yes 350 μL No Barcode Pack of 60
10565131 MultiSorp No 350 μL No Barcode Pack of 60
10449672 PolySorp No 350 μL No Barcode Pack of 60
10190825 Universal Binding No 330 μL No Barcode Pack of 50
10130665 Enhanced Binding Yes 330 μL No Barcode Pack of 50
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 13 options available.
13 options
Each lot is functionally tested for IgG binding.
Product Code. 10547781 Supplier Thermo Scientific™ Supplier No. 442404
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Each lot is functionally tested for IgG binding.

Description

Choose from four binding surfaces of these 96-well plates for reliable and reproducible results in colormetric assays.

Choose from four binding surfaces for reliable and reproducible results in colormetric assays with Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, which feature Thermo Scientific™ Nunc™, Immulon™ and Microtiter™ clear plates that are ideal for quantitative and qualitative solid phase immunoassays.

96-Well Solid Plates:

  • Clear polystyrene
  • Choice of four surfaces for optimal binding
  • Alphanumerically labeled rows and columns

Surfaces:

  • Thermo Scientific PolySorp, Immulon 1B, and Universal Binding (UB) are for adsorption of hydrophobic molecules.
  • Thermo Scientific Medisorp and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules.
  • Thermo Scientific MaxiSorp, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays.
  • Thermo Scientific MultiSorp is very hydrophilic and works well for glycans and water soluble proteins.

Ordering Information:

Please note that availability may vary by country. 467320, 456537, 460984 and 439454 include certificate

TRUSTED_SUSTAINABILITY

Specifications

Barcode No Barcode
Binding Property Hydrophilic
Certified No
Format Standard
Lid Without Lid
Detection Method Colorimetric
Array 8 x 12
Packaging 60 Cases
Color Clear
Product Type Microplate
Material Polystyrene
Volume (Metric) Well 400 μL
No. of Wells 96
Volume (Metric) Working 350 μL
Sterility Non-sterile
Surface Treatment MaxiSorp
Well Shape Round, Flat Bottom (F-well)
No. per Case 60
No. per Pack 5
Plate Blocking None
Quantity 60/Cs.
Target Molecule Antibodies (Glycoproteins)
Well Design F96
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Frequently Asked Questions (FAQs)

What is a good alternative to Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, MaxiSorp, 350 µL (Cat. No. 439454)?

There is no exact alternative. The closest alternative can be found on the same product page, Cat. No. 442404. Two other potential alternatives on the same product page are Cat. No. 456537 and Cat. No. 460894. You can view the specific differences between these products on the product page by clicking into the "Full Specifications" dropdown for each.

Is there an alternative to Nunc MaxiSorp flat-bottom 96 well plates (Cat. No. 44-2404-21)?

We recommend Thermo Scientific Clear Flat-Bottom Immuno Nonsterile 96-Well Plates (Cat. No. 442404) as an alternative to the discontinued Nunc Nunc MaxiSorp flat-bottom 96 well plates.
For Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, is the surface treatment solely on the bottom of the well, or is it also applied to the sides?

For energy treated plates, the entire plate surface receives the energy treatment. The MaxiSorp, PolySorp, MediSorp and MultiSorp plates are energy treated plates. For traditionally treated plates, the well surface is treated up to the listed working volume.
What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?

We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.

Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?

Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.
Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

What is the difference between certified and non-certified MaxiSorp plates and modules?

Both of these surfaces are identical. The only difference between them is that for the certified plates, a representative sample from each manufacturing lot undergoes a Binding Capacity test. This test is an ELISA-like assay used in our quality control laboratories to ensure binding capabilities.
How can I reduce high background readings and non-specific binding, when performing ELISA using Nunc-Immuno MaxiSorp Plates?

Assay sensitivity depends strongly on efficient removal of non-specific reacting molecules. High background readings and coating instability can be minimized by addition of a blocking step after the first coating. The excess surface is then occupied by indifferent molecules.
We recommend washing three times after each coating step by using a solution of 0.15 M phosphate buffer (pH 7.2) with 0.2 M NaCl and 0.05% Tween 20.
For blocking, we recommend using 0.5% BSA, 1% casein or 1% gelatin in 0.15 M phosphate buffer (pH 8.2) or carbonate buffer (pH 9.6).

Which surfaces do you recommend for RIA (radioimmunoassay)?

The choice of plates or modules will depend on the type of RIA you are planning to perform and specific assay conditions. Nunc provides two different surfaces for immunoassays, PolySorp and MaxiSorp.
- MaxiSorp surface is highly charged and should be used if the assay requires quantitative measurement of proteins (antibodies) or molecules with polar groups.
- PolySorp surface should be used if the assay consists of less polar molecules with hydrophobic characteristics.
Which 96-well Immuno plates or modules do you recommend for various applications?

The following list offers a brief description of the features of Nunc products and their specific applications:
- Nunc-Immuno Plates, MaxiSorp surface - These plates are designed for solid phase immuno assays and have a polystyrene surface with high affinity for polar groups and hydrophilic molecules. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Plates, PolySorp surface - These plates have a polystyrene surface which adsorps less polar molecules compared to the MaxiSorp surface and has a high affinity for hydrophobic groups. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Modules - These modules are designed for solid-phase immuno assays. The modules are available in 8-, 12- or 16-well formats with (F), (U), and (C) bottom-well and 8-well BreakApart with (C) bottom-well designs. These different formats allow one to choose the style which is appropriate for their assay design.
BreakApart Modules and LockWell Modules can be used for radioimmunoassays.
- Nunc StarWell Immuno Breakable Modules - The 8-well modules feature eight fins on the inner wall of the (C) bottom wells. This design increases the surface area by 50%. The increase in surface area allows more molecules to be immobilized, increasing assay signal. The fin configuration provides shorter diffusion distance to the surface, reducing incubation times.
- LockWell Immuno Breakable Modules - Each plate consists of 1 x 8 breakable strips. These strips are assembled in a designed frame which locks each well into place by a spring lock. This spring lock design orientates each well at the same horizontal level allowing uniform washing and reading. The LockWell Modules are available with round (U), (C) or StarWell bottom-well designs.
- Plates and Modules with (CovaLink) Covalent Binding Surfaces: These plates can be used to bind proteins, peptides, DNA or carbohydrates. Covalent linkage occurs via secondary amine group. This allows a specific orientation and provides improved stability compared to passive adsorption due to chemical binding between molecules. Binding of molecules on PolySorp and MaxiSorp surfaces is obtained by passive adsorption. CovaLink is a surface grafted with secondary amino groups (<NH) which serves as a bridge for covalent coupling. The covalent binding can immobilize small molecules such as biotin or peptides which may otherwise bind weakly by physical adsorption and facilitate the recognition by detection molecule.
- Nunc FluoroNunc/LumiNunc 96-Well Plates: These plates are optimized for IFMA (Immunofluorometric Assays) or FIA (Fluorometric Immuno Assays). The transparent polystyrene plates give a low background fluorescence and are optimal where a read-through system is used. The white plates/modules provide maximum reflection of fluorescence signal while maintaining low background. The white plates/modules are often used for epifluorescence reading. Black modules reduce background fluorescence and minimize back-scatter light which is often encountered in epifluorescence.

What is the maximum binding capacity for proteins on the Thermo Scientific MaxiSorp surface and PolySorp surface?

Molecules bind to our PolySorp and MaxiSorp surfaces through passive adsorption.
Using IgG as a reference molecule and knowing that it is a globular molecule, theoretical calculations indicate that the maximum binding for proteins are:
- For the MaxiSorp surface, in monolayer: 650 ng/cm^2
- For the PolySorp surface: 220 ng/cm^2.
What is the difference between your Thermo Scientific PolySorp, MaxiSorp, and MiniSorp surfaces?

MaxiSorp, PolySorp and MiniSorp surfaces were developed for immunology assays:
- The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity for molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. Thus, it offers very high sensitivity in double antibody "sandwich" tests.
- The PolySorp surface is more hydrophobic than the MaxiSorp surface. It has high affinity to molecules of a more hydrophobic character. This surface is particularly suited to non-protein antigens including virus antigens.
- The MiniSorp surface is a polyethylene surface with very low affinity to molecules of any type. This type of surface is ideal for the liquid phase immuno techniques.
- The MiniSorp surface is only available in a tube format, while the MaxiSorp and PolySorp surfaces are offered in both 96-well plate and module formats.
Which Nunc immunodiagnostic passive surface should I choose: PolySorp, MediSorp, MaxiSorp, or MultiSorp?

Our passive immunodiagnostic surfaces are based on a series of treatments that modify the polystyrene surface to different final levels of hydrophilicity. Selection of one of these surfaces for ELISA development should be based on the characteristics of the biomolecule you seek to immobilize. PolySorp is fairly hydrophobic and should be used for biomolecules that have hydrophobic domains, e.g., lipids, lipoproteins, and large proteins. MediSorp is slightly hydrophilic and can be used for biomolecules with hydrophilic and hydrophobic properties, e.g., medium-to-large proteins such as albumin, or amphiphilic biomolecules such as LPS. MaxiSorp is quite hydrophilic, optimized for binding of IgG (and any other molecule with similar characteristics) and thus is a popular choice for sandwich ELISA and similar protocols. MultiSorp is very hydrophilic, and in turn will immobilize very hydrophilic biomolecules such as glycoproteins. Some optimization will always be necessary, and in many cases screening for binding optimization may involve a few surface types.

What is the difference between a certified and an uncertified immunodiagnostic plate, e.g., Cat. No. 439454 vs. 442404?

There is no difference in the manufacturing process. Certified plates are performance tested (using an antibody binding protocol) during quality control, whereas uncertified plates are not. This test is expensive for us to perform, so we offer the uncertified version at cost savings customers for whom antibody binding testing results are not critical.

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