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Thermo Scientific™ DH5α Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Brand: Thermo Scientific™ EC0112
100.01 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
- The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
- RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA
- High transformation efficiency: >1x109 cfu/μg pUC19 DNA
- Suitable for routine and high-throughput cloning applications
- Genetic markers that allow for blue/white colony screening
- Convenient two reactions per vial packaging
- S.O.C. Outgrowth medium included
- DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
- Efficient DNA cloning derived from PCR, cDNA-generation reactions
- recA1 marker facilitates working with difficult to transform DNA
- Ideal for generation of cDNA libraries using plasmid-derived vectors
- Site-directed mutagenesis
- Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
Specifications
| Chemically Competent | |
| Dry Ice (-80°C) | |
| E. coli | |
| Cloning |
| Lacks F' Episome | |
| DH5α | |
| Tube | |
| 20 Reactions |