Competent Cells
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Invitrogen™ MAX Efficiency™ DH5α Competent Cells
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Gibco™ FreeStyle™ CHO Expression Medium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Animal origin-free, chemically defined, protein-free medium
| Cell Line | FreeStyle™ CHO-S Cells, CHO |
|---|---|
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Product Type | Expression Medium |
| Endotoxin Level | ≤0.25 EU/mL, ≤ 0.25 EU/mL |
| Classification | Chemically Defined, Protein-Free, Serum-Free |
| Culture Type | Suspension Cell Culture |
| Phenol Red Indicator | No Phenol Red |
| Product Line | Freestyle, Gibco |
| Serum Level | Serum-Free |
| Glutamine | No Glutamine |
Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.
Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli
One Shot BL21 Star (DE3)pLysS Chemically Competent E. coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET.
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Invitrogen™ One Shot™ PIR2 Chemically Competent E. coli
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
Invitrogen™ MAX Efficiency™ DH10B Competent Cells
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli
One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | Not suitable for cloning unstable DNA |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Promoter | Trc, Tac, Lac |
| Preparing Unmethylated DNA | No |
| Reduces Recombination | Yes (recA1) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptamycin, Tetracycline) |
| Format | Tube |
| Product Type | Chemically Competent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | Yes |
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | No |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | May be used for plasmids >20 kb |
| Reduces Recombination | Yes (recA13) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Electrocompetent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | No |
Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells
ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency.
Invitrogen™ Library Efficiency™ DH5α Competent Cells
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.
Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.
Invitrogen™ ElectroMAX™ DH10B Cells
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.
Thermo Scientific™ BL21(DE3) Competent Cells
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.