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Gibco™ MEM α, no nucleosides
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Product Code. 12509069
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Product Code. 12509069 Supplier Gibco™ Supplier No. 12561056
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Description

Requires

Supplementation

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Phenol Red, L-glutamine

Without: Ribonucleosides, Deoxyribonucleosides

Using DMEM α

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1X
Form Liquid
Product Type MEM α (Minimum Essential Medium α)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives No HEPES, No Ribonucleosides, No Deoxyribonucleosides
For Use With (Application) Mammalian Cell Culture
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 1 x 500 mL
Shipping Condition Ambient
Format Bottle
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Culture Environment CO2
Culture Type Mammalian Cell Culture
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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