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  5. Novus Biologicals™ IL-1 alpha/IL-1F1 Antibody Pair [HRP]

Novus Biologicals™ IL-1 alpha/IL-1F1 Antibody Pair [HRP]

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18205978 15 Plates Pack of 15
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Product Code. 18205978 Supplier Novus Biologicals™ Supplier No. NBP27952215PLATES

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Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-1 alpha/IL-1F1.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 6.25 pg/mL
Assay Sensitivity 6.25-400 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-1 alpha/IL-1F1.
Target Species Mouse
For Use With (Application) Sandwich ELISA
Gene Alias Hematopoietin-1, IL1, IL-1 alpha, IL-1A, IL1-ALPHA, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, preinterleukin 1 alpha, pro-interleukin-1-alpha
Gene ID (Entrez) 3552
Gene Symbol IL1A
Host Species Rabbit/Mouse
Kit Contents
  • Standard: Each vial contains 30 ng of recombinant Mouse IL-1 alpha/IL-1F1.
  • Capture Antibody: 0.5 mg/mL of mouse anti-Mouse IL-1 alpha/IL-1F1 monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL of rabbit anti-IL-1 alpha/IL-1F1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
    		•
    Quantity 15 Plates
    Regulatory Status RUO
    Research Discipline Apoptosis, Biologically Active Proteins, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
    Storage Requirements Storage is content dependent.
    Target IL-1 alpha/IL-1F1
    Technique 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
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