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  5. Novus Biologicals™ Leptin/OB Antibody Pair [HRP]

Novus Biologicals™ Leptin/OB Antibody Pair [HRP]

Product Code. p-7183219 Shop All Bio Techne Products
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Quantity:
15 Plates
5 Plates
Unit Size:
Pack of 15
Pack of 5
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Product Code. 18232097

Brand: Novus Biologicals™ NBP27953815PLATES

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Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Leptin/OB.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 7.81 pg/mL
Assay Sensitivity 7.81-500 pg/mL
Assay Sandwich ELISA
Conjugate HRP
Product Type Antibody Pairs
Specificity This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Leptin/OB.
Target Species Human
For Use With (Application) Sandwich ELISA
Gene Alias FLJ94114, leptin, leptin (murine obesity homolog), leptin (obesity homolog, mouse), Obese protein, obese, mouse, homolog of, Obesity factor, OBOBS
Gene ID (Entrez) 3952
Gene Symbol LEP
Host Species Mouse
Kit Contents
  • Standard: Each vial contains 172 ng of recombinant Human Leptin/OB.
  • Capture Antibody: 0.5 mg/mL of mouse anti-Human Leptin/OB monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL of mouse anti-Human Leptin/OB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
    		•
    Quantity 15 Plates
    Regulatory Status RUO
    Research Discipline Biologically Active Proteins, Cytokine Research, Diabetes Research, Immunology, Lipid and Metabolism
    Storage Requirements Storage is content dependent.
    Target Leptin/OB
    Technique 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
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