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Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Brand:  Gibco™ 12634028

299.25 EUR valid until 2024-04-30
Use promo code "21741" to get your promotional price.



Additional Details : Weight : 6.34000kg

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Product Code. 11550446

  • 315.00 € / Pack of 10
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Description

Description

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin
Liquid
Reduced Serum
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Sustainable packaging
Gibco™
Room Temperature
MRC-5, SP2, Vero, WI-38 and Jurkat
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
No Glutamine, No HEPES
cGMP-compliant under the ISO 13485 standard
10 x 500 mL
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For Research Use Only. Not for use in diagnostic procedures.