Gibco™ MEM, HEPES, GlutaMAX™ Supplement

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

• Available with Earle's salts for use in CO₂ incubator, or with Hank's salts for use without CO₂
• Gibco MEM with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
• Modified as follows: with GlutaMAX™, HEPES, phenol red
• Made with Earle's salts
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Sustainable packaging

Cell Culture, Mammalian Cell Culture