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Invitrogen™ MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), NovaFluor™ Yellow 570, eBioscience™
Mouse Monoclonal Antibody
176.00 € - 339.00 €
Specifications
| Antigen | MHC Class I (H-2Kd/H-2Dd) |
|---|---|
| Clone | 34-1-2S |
| Concentration | 0.1 mg/mL |
| Content And Storage | 4°C, store in dark, DO NOT FREEZE! |
| Applications | Flow Cytometry |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
30564829
|
Invitrogen™
M043T02Y01-A |
25 μg |
176.00 €
25µg |
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|
30564826
|
Invitrogen™
M043T03Y01-A |
100 μg |
339.00 €
100µg |
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Description
Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested: This 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.4 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest Master mixes. Whole Blood compatibility: When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.
This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.Specifications
| MHC Class I (H-2Kd/H-2Dd) | |
| 0.1 mg/mL | |
| Flow Cytometry | |
| NovaFluor Yellow 570 | |
| Mouse | |
| RUO | |
| PBS with BSA and 0.09% sodium azide; pH 7.2 | |
| P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428 | |
| 14964, 14972 | |
| Primary | |
| Affinity chromatography |
| 34-1-2S | |
| 4°C, store in dark, DO NOT FREEZE! | |
| Monoclonal | |
| Liquid | |
| IgG2a κ | |
| Mouse | |
| H2-D1 | |
| class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein | |
| H2-D1, H2-K1 | |
| Antibody |
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