Invitrogen™ 5-CFDA, AM (5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester)

• Measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product
• Electronically neutral AM ester of CFDA can be loaded into cells at lower concentrations than CFDA
• Upon hydrolysis by intracellular esterases, this AM ester also yields carboxyfluorescein: As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells

Cell Adhesion, Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Proliferation, Cell Tracing and Tracking, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Cellular Toxicology Assays, Chemotaxis and Cell Migration, Drug Discovery and Development, General Cell Tracing, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Ionic Homeostasis and Signaling, Target-Based ADME/Tox Assays, pH Detection