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Macherey-Nagel™ NTB Binding Buffer
43.75 € - 152.00 €
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
11952282
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Macherey-Nagel™
740595.150 |
150 mL |
43.75 €
150mL |
Estimated Shipment: 07-05-2024 Log in to see stock. |
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12788482
|
Macherey-Nagel™
740595.1 |
1000 mL |
152.00 €
1000mL |
Estimated Shipment: 08-05-2024 Log in to see stock. |
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Description
NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.- Silica matrix technology
- Fragment size: 20bp to 20kb
- Sample material: 20mg to 600mg agarose gel
- Elution volume: 20μL to 50μL
- Binding capacity: 6μg/10μL suspension
- Typical Recovery: 50% to 90%
- Preparation time: 60min/6 preps
- Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.