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  5. Thermo Scientific™ Pierce™ Crossflow Filtration Cassettes, 3–100 kDa MWCO

Thermo Scientific™ Pierce™ Crossflow Filtration Cassettes, 3–100 kDa MWCO

Product Code. 18091673 Shop All Thermo Scientific Products
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Product Code. 18091673 Supplier Thermo Scientific™ Supplier No. A57930

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Description

Pierce Crossflow Cassettes are membrane-based devices designed for concentrating and diafiltration of sample solutions such as proteins, lipids, viruses, and cell culture supernatants with volumes from 0.50–3 L. Suited for manufacturing and pilot-scale tangential flow filtration processes.

Pierce Crossflow Cassettes are designed for easy setup, flexibility, and robust performance. They can process sample solutions ranging from 0.5 to 3 liters in volume and operate via a peristaltic pump pressure-driven process where the sample solution containing solutes are pushed tangentially across the surface of a specialized Polyethersulfone (PES) membrane with low protein binding characteristics. The membrane surface per device is 200 cm2 and is offered in five different molecular-weight cutoffs (MWCOs) of 3, 10, 30, 50, and 100 kDa, providing a range of options to suit various needs.

Thermo Scientific Pierce Crossflow Filtration Cassettes are membrane-based devices designed for small-volume manufacturing and pilot-scale tangential flow filtration (TFF) of biological samples. They feature easy setup, optimal flexibility, and robust performance. These cassettes can process sample solution volumes of 0.5–3 liters and operate via a peristaltic pump pressure-driven process where the sample solution is pushed tangentially across the surface of a specialized polyethersulfone (PES) membrane.

Features of Pierce Crossflow Filtration Cassettes include:
• Designed for small-volume manufacturing and pilot-scale TFF applications
• Reusable without membrane replacement
• Fully assembled with easy setup requiring no holder
• High flow rate and sample recovery
• Low cost of operation

Pierce Crossflow Filtration Cassettes contain a PES membrane with low protein binding characteristics and have a specialized channel design that ensures high flux without optimization. The membrane has a surface area of 200 cm2 and the option of five different molecular-weight cutoffs (MWCOs): 3, 10, 30, 50, and 100 kDa. The membrane has small pores, preventing the passage of larger molecules like proteins that are retained in the flow feed path while smaller molecules like salts pass through, causing a decrease in solution volume and an increase in macromolecule concentration.

Pierce Crossflow Filtration Cassettes are easily cleaned and reusable. Each cassette can concentrate up to 1 liter of sample by a factor of >50 in 30 minutes or less. Two cassettes can be connected and run in parallel using a Y connector to scale and speed up the process. In this way, up to 6L of sample can be processed.

Each cassette includes all necessary accessories required for running the cassette with a laboratory peristaltic pump and a size 16 pump head. To make the buffer exchange process more convenient, a sealed diafiltration reservoir accessory can be used to help speed up the process.

Pierce Crossflow Filtration Cassettes are designed for small-volume manufacturing and pilot-scale TFF applications in diverse biological and biopharmaceutical processes, such as concentration and diafiltration of proteins, lipids, viruses, colloids, and cell culture supernatants.  The cassettes deliver efficient performance due to their high crossflow speed and low pump speed requirement.

TRUSTED_SUSTAINABILITY

Specifications

Filter Type Membrane Filter
Format Cassette
For Use With (Equipment) Labscale TFF system
Material Polypropylene
Material (Membrane) PES
MWCO 3 kDa
Pore Size 1.2 nm
Sterility Non-sterile
Fit for Purpose Certified Quality

Frequently Asked Questions (FAQs)

Are Pierce Crossflow Filtration Cassettes GMP grade?

Pierce Crossflow Filtration Cassettes are not GMP grade.
Can I run Pierce Crossflow Filtration Cassettes without the pressure gauge?

A pressure gauge is provided with every Pierce Crossflow Filtration Cassette. Pierce Crossflow Filtration Cassettes can be run without a pressure gauge; however, it is important to limit the flow rate to 200-300 mL/min to prevent tearing and potential collapse of the tubing. The flow rate can be determined by measuring the volume flow over 1 min. Alternatively, a pump speed of 300-350 rpm or a pressure of 1-1.5 bar will maintain a flow rate of 200-300 mL/min.
What are the factors that can speed up or slow down the rate of concentration or diafiltration?

High protein concentration and/or presence of particulate in the sample can slow down the rate of concentration/diafiltration. Adsorption of the sample to the membrane and lower temperature (4 degrees C vs room temperature) can also slow down concentration/diafiltration. Filtering your sample prior to processing with a crossflow filtration cassette will help speed up concentration/diafiltration.
What are the advantages of using a crossflow ultrafiltration device vs. a stirred cell device?

Crossflow ultrafiltration and stirred cell devices both effectively concentrate biomolecules with similar sample recoveries; however, a crossflow filtration device concentrates sample much faster than a stirred cell device.
Why should I use crossflow filtration instead of dialysis or chromatography methods?

Crossflow filtration is much faster for concentration and diafiltration than dialysis and chromatography methods.
What are the general applications of crossflow ultrafiltration?

Crossflow ultrafiltration in the biotech industry is typically for the following applications:
- Virus concentration
- Concentration of proteins in cell culture supernatant
- Diafiltration (desalting/buffer exchange) of protein or virus preparations
Are Pierce Crossflow Filtration Cassettes endotoxin-free?

Pierce Crossflow Filtration Cassettes are not endotoxin-free. The filtration cassettes can be flushed with 0.5 M NaOH to remove pyrogens (endotoxins).
Can I use Pierce Crossflow Filtration Cassettes to concentrate nucleic acid samples?

Pierce Crossflow Filtration Cassettes can be used to concentrate double-stranded and single-stranded nucleic acids.
When using a Pierce Crossflow Filtration Cassette, how can I reduce protein degradation during concentration?

To reduce protein degradation during concentration, choose a buffer and pH that are compatible with your protein. If necessary, perform buffer exchange into a more compatible buffer using a diafiltration reservoir. If your protein is temperature sensitive, perform concentration at 4 degrees C. Lastly, add protease inhibitors to your sample if your protein is susceptible to degradation.
I'm using a Pierce Crossflow Filtration Cassette. How do I prevent the Feed Line from adhering to the sample reservoir while the system is running?

To prevent the open end of the Feed Line from adhering to the wall of the sample reservoir, connect the Tube Reducer to the end of the Feed Line that will be submerged in the reservoir. Alternatively, perform an angled cut on the end of the Feed Line leaving an uneven opening.
What is the Tube Reducer used for, when using Pierce Crossflow Filtration Cassettes?

The Tube Reducer is used to connect size 16 tubing to size 15 tubing. This can be useful if you have a peristaltic pump with a size 15 pump head.
Is the Pierce Crossflow Filtration Cassette a walk-away device?

We do not recommend leaving the Pierce Crossflow Filtration Cassette running, unsupervised, for an extended period of time, especially when nearing the desired concentration volume. We recommend being near the device and/or periodically checking that the feed line is submerged in the sample and that there is no loss of flow rate due to collapsed tubing or tubing adhered to the side of the reservoir.
Can I use Pierce Crossflow Filtration Cassettes for protein fractionation?

Pierce Crossflow Filtration Cassettes can be used for protein fractionation, although separation will not be 100%. We recommend selecting a filtration cassette with an appropriate molecular weight cut-off (MWCO) that will separate target proteins (e.g., to separate a 150 KDa protein from a 15 kDa protein, use a cassette with a 50 kDa MWCO). For efficient separation of two proteins, ensure that there is at least a 10-fold difference in molecular weight (MW).
Can I use Pierce Crossflow Filtration Cassettes to remove cells and cell debris from cell culture media?

We do not recommend using Pierce Crossflow Filtration Cassettes for removal of cells and cell debris. Using the filtration cassette for this purpose will slow down the concentration time and potentially impact recovery. Instead, prior to concentration of the cell culture supernatant, we recommend centrifuging and filtering the supernatant to remove cells and cell debris.
Can I use a Pierce Crossflow Filtration Cassette to concentrate virus?

Pierce Crossflow Filtration Cassettes can be used to concentrate virus. We recommend using Pierce Crossflow Filtration Cassettes, 3-100 kDa MWCO (Cat. No. A57934) for this purpose.
What can I use to purify my antibody after concentration with the Pierce Crossflow Filtration Cassette?

Concentrated antibody can be purified using protein A supports, e.g., Pierce High-Capacity Protein A MagBeads, alkali stable (Cat. No. A53035, A53036, A53037, A53038), or MabCapture C High-Capacity Protein A Resin (Cat. No. A53031, A53033, A53034). Alternatively, for IgG1 antibody species, we recommend using Pierce Protein A/G Magnetic Agarose Beads (Cat. No. 78609, 78610) or Pierce Protein A/G Plus Agarose (Cat. No. 20423, 20424). For more information regarding binding characteristics of antibody-binding proteins, please refer to Tech Tip #34 (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).
Can I reuse Pierce Crossflow Filtration Cassettes?

Pierce Crossflow Filtration Cassettes can be reused up to 3 times. Successful reuse of Pierce Crossflow Filtration Cassettes is sample-dependent. We recommend using the same sample type for best results.
Can the pump tubing supplied with the Pierce Crossflow Filtration Cassette be reused?

The tubing supplied with the Pierce Crossflow Cassette can be reused. To reduce stress on the tubing and maintain it in good working condition, maintain the pressure at 1-1.5 bar using the pressure gauge provided. Replacement tubing can be obtained by purchasing the Crossflow Filtration Accessory Pack (Cat. No. A57937).
How is the Crossflow Y-Connector (Cat. No. A57936) used with Pierce Crossflow Filtration Cassettes?

In order to filter sample volumes larger than 3 liters (up to 6 liters), two Pierce Crossflow Filtration Cassettes need to be used. The Crossflow Y-Connector (Cat. No. A57936) is used to connect the sample feed line into the two Pierce Crossflow Filtration Cassettes.
I'm using a Pierce Crossflow Filtration Cassette. How do I perform buffer exchange without a Pierce Crossflow Diafiltration Reservoir (Cat. No. A57935)?

To perform buffer exchange manually, we recommend following this procedure:

  1. Prepare Exchange Buffer that is 5X the volume of your sample.
  2. Set up your Pierce Crossflow Filtration Cassette as stated in the user manual.
  3. Turn on the pump and concentrate your sample until the volume is reduced ~90%.
  4. Dilute the concentrated sample to the original volume with Exchange Buffer.
  5. Repeat steps 3 and 4 until the desired solute removal has been achieved.
Note: Precipitation can occur at high concentration for some proteins. If this occurs, reduce sample volume by a lesser percentage in each step and increase the number of repeats.
What additional items are required for using Pierce Crossflow Filtration Cassettes?

All of the required tubing and a pressure gauge are included with the Pierce Crossflow Filtration Cassette. Additional items required, but not provided, include a peristaltic pump and a pump head.
How do Pierce Crossflow Filtration Cassettes compare to Tangential Flow Filtration (TFF)?

Pierce Crossflow Filtration Cassettes work by Tangential Flow Filtration (TFF), which is also known as crossflow filtration. It involves the passage of fluid parallel to the filter rather than directly through it. The latter can cause the filter to clog and slow down the concentration process.
Can I autoclave Pierce Crossflow Filtration Cassettes?

We do not recommend autoclaving Pierce Crossflow Filtration Cassettes. High temperatures will significantly increase the membrane molecular weight cut-off (MWCO). To sterilize Pierce Crossflow Filtration Cassettes, use a 70% ethanol solution.
What percent recovery of my target molecule can I expect with Pierce Crossflow Filtration Cassettes?

More than 98% recovery of your target molecule can be achieved by performing a low-volume buffer rinse through the tubing and cassette following sample concentration.
Does Thermo Fisher Scientific offer pumps for use with Pierce Crossflow Filtration Cassettes?

We recommend using a Masterflex pump and pump head that can be purchased through Fisher Scientific or from other major suppliers.
What pump and pump head should I use with the Pierce Crossflow Filtration Cassette?

Any pump and pump head that can deliver a flow rate of 200-300 mL/min can be used. The pump head must be compatible with size 16 tubing for use with 1 filtration cassette, and size 15 tubing for use with 2 filtration cassettes. We recommend using a Masterflex pump and pump head that can be purchased through Fisher Scientific or from other major suppliers.
How do I select the best Pierce Crossflow Filtration Cassette for my sample?

For maximum protein recovery, samples should have a molecular weight that is two-fold higher than the molecular weight cut-off (MWCO) of the filtration cassette.
What membrane is used in the Pierce Crossflow Filtration Cassettes?

The Pierce Crossflow Filtration Cassettes contain a polyethersulfone (PES) membrane. Each cassette has a volume capacity of 0.5 to 2.5 L with 3 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa molecular weight cut-offs (MWCOs).
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