Invitrogen™ PPAR gamma Polyclonal Antibody

Description

PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits. PA3-821A has been successfully used in IF and Western blot procedures. Previous batches of this antibody have been used in gel shift, immunocytochemistry, and immunofluoresence procedures. By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches gives predominant nuclear staining. The PA3-821A immunizing peptide corresponds to amino acid residues 284-298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1, but exhibits no significant homology with PPAR alpha or NUC1. This sequence is completely conserved in mouse, human, and rat.

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.