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Ambion™ Silencer™ Select Pre-Designed siRNA
Description
Silencer Select Predesigned siRNAs offer researchers a powerful and reliable tool for gene silencing applications. Designed with advanced algorithms to ensure high specificity and efficiency, these siRNAs offer a reliable and powerful solution for gene silencing applications. Chemically modified with locked-nucleic acid (LNA) modifications to enhance stability and reduce off-target effects, Silencer Select Predesigned siRNAs deliver consistent and reproducible results across a wide range of experimental conditions.
Key features include:
- Precise silencing—minimize off-target effects with the combination of algorithm design and LNA chemical modifications
- Enhanced potency—achieve effective gene silencing with lower siRNA concentrations
- Guaranteed silencing—two out of two predesigned siRNAs will result in gene knockdown
- Comprehensive support—access detailed protocols and expert technical support
Optimized design for enhanced specificity
Our internally developed algorithm has been fine-tuned to create siRNAs with sequences that maximize on-target effects while minimizing off-target interactions. This optimized design approach significantly reduces the risk of unintended gene silencing, thereby increasing the reliability and interpretability of experimental results. The specificity of Silencer Select siRNAs allows researchers to confidently attribute observed phenotypes to the silencing of their gene of interest.
Effective silencing at lower concentrations
A key advantage of Silencer Select siRNAs is their ability to achieve effective silencing at lower siRNA concentrations. This not only reduces experimental costs but also minimizes the potential for off-target effects that can occur at higher doses. Maintaining high knockdown efficiency at reduced concentrations highlights the potency and precision of our siRNA design.
Streamlined workflow for rapid results
Utilizing our gene knockdown technology is straightforward and efficient. Simply order an siRNA for the desired gene target, deliver it to the cells, and detect knockdown efficiency and validate the resulting phenotype. Take advantage of our validated siRNA designs for better confidence in your experiments. This streamlined process allows for rapid experimental progress and accelerated discovery in gene function studies.
Specifications
Specifications
| Content And Storage | Contains dried siRNA (20 nmol) and Nuclease-free Water (1.75 mL), shipped at ambient temperature. Upon receipt, store siRNAs in a non-frost-free freezer at or below –20 °. |
| Description | Silencer™ Select Pre-Designed siRNA, 20nmol, Standard Purity, For RNAi, epigenetics and Non-Coding RNA research, RNAi, siRNA |
| RNAi Type | siRNA |
| Final Product Type | Silencer™ Select pre-designed siRNA |
| For Use With (Application) | RNAi, epigenetics and non-coding RNA research, RNAi, siRNA |
| Product Line | Silencer Select, Ambion |
| Product Type | Silencer™ Select pre-designed siRNA |
| Purity | Standard purity |
| Quantity | 20 nmol |
| Sample Size | 20 nmol |
Frequently Asked Questions (FAQs)
Yes, our Silencer Select siRNA are designed to target both cytoplasmic and nuclear long (>100 nt) ncRNAs for human, mouse and rat. ncRNAS for which pre-designed siRNAs are available closely match the TaqMan Non-coding RNA assays we offer.
We recommend diluting it 1:10 to obtain a 10 µM working solution.
We recommend adding 50 µl water to 5 nmol of lyophilized siRNA to obtain a 100 µM stock solution that can be stored.
Silencer Select siRNA resuspended at concentrations ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. We recommend storing the resuspended siRNA at -20 degrees C or -80 degrees C. If you plan to exceed 50 freeze-thaw cycles, we recommend making a few large aliquots after resuspension and limiting each aliquot to 50 freeze-thaws. Further, we recommend storing the aliquots that are not in use at -70 to -80 degrees C for long-term storage.
For Research Use Only. Not for use in diagnostic procedures.
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