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  5. Thermo Scientific™ SureSTART™ WebSeal™ 96-Well Microtiter Plates, Round U, V, and Flat-Bottom, Level 2 High-Throughput Applications

Thermo Scientific™ SureSTART™ WebSeal™ 96-Well Microtiter Plates, Round U, V, and Flat-Bottom, Level 2 High-Throughput Applications

Product Code. 15142729
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Total Volume (Metric) Well:
220 μL
270 μL
350 μL
No. per Pack:
10 Plates
100 Plates
Well Shape:
Round
Round, Conical Bottom (V-well)
Round, Flat Bottom (F-well)
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Product Code. Total Volume (Metric) Well No. per Pack Well Shape Barcode unitSize
15142729 220 μL 10 Plates Round, Conical Bottom (V-well) No Barcode Pack of 10
15132729 220 μL 100 Plates Round, Conical Bottom (V-well) No Barcode Pack of 100
15866261 220 μL 100 Plates Round, Conical Bottom (V-well) Includes Barcode Pack of 100
15122729 270 μL 10 Plates Round No Barcode Pack of 10
16383757 270 μL 10 Plates Round Includes Barcode Pack of 10
15112729 270 μL 100 Plates Round No Barcode Pack of 100
16592050 270 μL 100 Plates Round Includes Barcode Pack of 100
15806271 220 μL 10 Plates Round, Conical Bottom (V-well) Includes Barcode Pack of 10
15102729 350 μL 10 Plates Round, Flat Bottom (F-well) No Barcode Pack of 10
15192719 350 μL 100 Plates Round, Flat Bottom (F-well) No Barcode Pack of 100
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Product Code. 15142729 Supplier Thermo Scientific™ Supplier No. 60180P217
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Description

Use SureSTART™ WebSeal™ 96-Well Microtiter Plates, Round U, V, and Flat-Bottom, Performance Level 2, for high-throughput analysis. They are certified and conform to ANSI-SLAS 4-2004 standards.

Thermo Scientific™ SureSTART™ Level 2 WebSeal™ 96-well Round U, V, and Flat-Bottom Microtiter Plates are designed to ensure high quality data with an uninterrupted workflow for high-throughput GC and HPLC/UHPLC applications. They are ideal for regulated labs that use water and polar solvents.

Use Level 2 polypropylene well plates for routine QA/QC workflows, sensitive applications with lower sample concentrations, and data collection for total ion chromatograms (TIC).

These 96-well plates are certified to ensure autosampler compatible physical dimensions and conform to ANSI-SLAS 4-2004 standards. Seal these well plates with SureSTART WebSeal 96-Well Plate Sealing Mats, 7 mm Diameter, Round, Level 2.

Performance Level 2 Plates – for High Throughput Applications

  • Ideal for non-regulated labs that use water and polar solvents
  • Certified plastic well plates, seals, tapes
  • GC-MS and LC-MS tested
  • Suitable for HPLC/UHPLC applications
  • Suitable for QA/QC workflows and sensitive applications with low sample concentrations
  • Ideal for verifying compound presence and quantification using detection techniques such as UV, DAD/PDA, RI, Fluorescence, and ELSD
  • Specification Certified
  • All Thermo Scientific SureSTART WebSeal well plates are dimensionally verified

Lot Traceable

  • 100% traceable through the manufacturing process
  • Certificate of Conformance available by lot number upon request

About Thermo Scientific WebSeal Well Plates & Mats

Thermo Scientific WebSeal plates and mats are available in three performance levels:

  • Level 1 well plates are for everyday applications and are suitable for non-regulated labs using water and polar solvents
  • Level 2 well plates are for high-throughput applications and are suitable for regulated labs using water and polar solvents
  • Level 3 well plates are for high performance applications and are suitable for regulated labs using all solvent types including non-polar solvents
TRUSTED_SUSTAINABILITY

Specifications

Barcode No Barcode
Certified Certified, GC/MS and LC/MS Tested
Array 8 x 12
Product Line SureSTART WebSeal Level 2
Color Clear
Material Polypropylene
No. of Wells 96
Volume (Metric) Working 10 to 190 μL
Sterility Non-sterile
Surface Treatment Non-coated
Total Volume (Metric) Well 220 μL
Well Shape Round, Conical Bottom (V-well)
No. per Pack 10 Plates
Temperature Range (Metric) -80°C to 121°C
Height (Metric) 14.7 mm
Diameter (Metric) Well 7 mm
Plate Size Microtiter
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Frequently Asked Questions (FAQs)

Are bar-coded WebSeal plates available?

Yes, bar-coded WebSeal plates are available as a catalog product for the famly of Thermo Scientific Vanquish UHPLC machines only. However, plates can be provided with a pre-printed adhesive label applied. Contact your sales representative to assist with completing a form where you can define the bar code information and location of application onto the plate. Additional information required includes the physical requirements for the label such as temperature stability, solvent stability, etc.
What is the best way to seal a WebSeal Plate plus or plates with glass or PTFE inserts that need to be incubated at elevated temperatures?

WebSeal Plate+ or plates with glass or PTFE inserts should never be sealed with tapes or adhesive foils. Silicone mats should be used with the WebSeal Plate&plus products, preferably with a protective PTFE film to give increased chemical protection.

The glass inserted plates are sealed with specific silicone plug mats or individual caps. Again, most have additional PTFE films of layers to give increased chemical protection.

These plates, inserts, and mats were originally designed for combinatorial chemistry techniques with in situ chemical derivatization. Use with elevated temperatures is possible with the inserts and plugs. These are the closest plate format equivalent to glass autosampler vial products.

How do the Thermo Scientific-WebSeal Plate plus products compare to standard and certified products?

The WebSeal Plate+ coating provides additional surface protection that further reduces the possibility of interactions with solvents, sample component, or other materials. WebSeal Plate+ microptates display ultra-low background extractable organics profiles that compare favorably to our mass spectrometry certified vial products.
How do WebSeal-Certified low bleed plates compare to others?

Well plates are manufactured in molds. In order to ''force'' the ready produced plate to come out of this mold for the next shot, some manufacturers use release agents (organic compounds that act as lubricants). These release agents can be detected in your chromatogram. The grade of polypropylene used by most plate manufacturers can contain monomers or other added ingredients that are susceptible to leaching on contact with organic solvents. WebSeal-Certified products are manufactured from high purity resins and use a process that does not require mold release agents and that is why they exhbit no significant background extractables.

Do standard WebSeal plates bleed with organic solvents, or is it better to purchase certified plates?

If you are using high concentrations of organic solvents in your sample, then you may be concerned about ''bleed''. This is a term which normally implies a constant loss of material from the column or separation system which leads to high background levels, increased noise, and loss of sensitivity. If using solutions with a high level of buffer or water, then this should not be a concern as such components will not leach under these conditions. All plates may be used.

The evaluation of the plastic resins used in the production of plates was also carried out by controlled extraction of leachable components with organic solvents associated with HPLC and GC operation. These included methanol, acetonitrile, and hexane. Standard plates showed low level of extractables when tested at the highest instrument sensitivity range. These extractables found are characteristic of the common materials used for microplates in the clinical market and plates that have been used for general purpose chromatography applications. This indicates that for many non-critical applications and with higher concentrations of sample, the standard quality plates will be acceptable. These plates are termed Chromatography Tested.

The very low levels of background extractables found during our testing of some plates showed for applications run at more sensitive instrument settings. Where higher purity of the plate is required, you should directly switch to the Certified plates as they are the lowest extraction PP plates currently available on the world market. For critical analysis with high organic solvent concentrations and low sample concentrations, the Certified product range should be the plates of choice.
Are WebSeal products compatible with GC-MS?

When using polymeric sample containers for GC or GC-MS applications, it is always important to consider the impact of background extractable compounds on the analysis. The presence of plasticizers and nucleation agents in the plastic material is the main source of background contamination. Efforts have been made to use only those plastics which are low in such additives.

WebSeal silicone mat products have been extensively evaluated for premium performance with regards to purity. Most work done has been to detemine that the resins are chromatographically acceptable with MeOH, Acetonitrile, and n- Heptane. The conditions we used for creating extractable data (temperature, column, detector, sensitivity, etc.) are published on thermofisher.com/webseal. Review of the chromatographic data will assist you in determining the suitability of specific products. In many cases, we can recommend our products for GC as well as HPLC applications.
Do WebSeal plates work with protein and bio analysis matrix?

The WebSeal plates are not compatible with ELISA and other fluorimetic or luminescent measurements or assays. They are suitable for use as sample collection plates for peptides and other bio-molecules followed by separation on Bio-LC or SEC systems. If the final destination of the sample workflow is an HPLC system, then these are valid product choices. If used for non-chromatographic techniques, then other plate products may be more suitable.
What are the temperature limits for the WebSeal microplate products?

Polypropylene microplates and silicone mats will tolerate exposure to temperatures as low as -80 degrees C and may be stored in cryogenic conditions. Polypropylene microplates and silicone mats will tolerate exposure to temperatures as high as 121 degrees C for up to 15 mins for the purpose of sterilization by autoclaving. The upper temperature limit of these products for extended periods of time (up to 7 days of constant heating) is 100 degrees C.
Can Thermo Scientific WebSeal well plates and mats be used on an automated liquid handling system such as Tecan, Hamilton, and others?

Thermo Scientific Webseal microplate products adhere to the Society for Biomolecular Screening (SBS) microplate formats. Instruments from vendors such as Tecan, Hamilton, Gilson, PerkinElmer, and others can be used within the instrument guidelines.
Does Thermo Fisher Scientific have a compatibility table for autosamplers and WebSeal well plates and mats?

Yes, visit this page (https://assets.thermofisher.com/TFS-Assets/CMD/brochures/TG-Autosampler-Compatibility-Well-Plates-EN.pdf) for a list of chromatography autosamplers that are compatible with microplates.

What is the difference between the SureSTART Performance Level 1, Performance Level 2, and Performance Level 3 vials?

All glass vials found within the three different performance levels are made from Type 1 Class A Borosilicate glass. This type of glass has excellent chemical resistance properties that make it ideal for the containment or storage of acidic, neutral and alkaline samples/analytes. The vials also have high temperature resistance and are suitable for use at temperatures between -50 degrees C to 120 degrees C (although a rapid change in temperature of the products should be avoided).

The Performance Level 1 vials are designed to identify those which are suitable for simple routine, testing with limited budget. Performance Level 1 glass vials have a single wavy line.

For most analytical requirements, the Performance Level 2 products will be suitable for use with higher sensitivity methods while maintaining throughput. Performance Level 2 glass vials have two wavy lines.

For the most stringent analytical requirements, the Performance Level 3 products have advanced features which make them suitable for use with difficult samples in terms of chemistry and available sample volume. All Performance Level 3 products are made from 33-expansion glass and these vials have three wavy lines.
What happened to National, Chromacol, and other brands for chromatogenic workflow accessories?

Many of the products that were branded as National Scientific and Chromacol have been absorbed into the new Thermo Scientific SureSTART Collection, although there are still a few products that still exist within the marketplace.

For more information, please contact your local sales representative or complete the inquiries form at the bottom of the SureSTART Autosampler Vials and Caps page (https://www.thermofisher.com/us/en/home/industrial/chromatography/chromatography-consumables/autosampler-vials-caps-hplc-gc.html).
What is the SureSTART products portfolio for chromatographic workflows?

The SureSTART portfolio is a comprehensive list of vials, caps, inserts, well plates, mats and accessories that are suitable for use with chromatographic workflows. The SureSTART portfolio contains three different performance levels that have been quality checked to provide a robust and reliable product.

The SureSTART portfolio includes products that were previously found in our legacy brands such as National Scientific, Chromacol, and Sun-Sri.
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