missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. 15306934

Invitrogen™ Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)

Product Code. 15306934
Change view
Click to view available options
Quantity:
100 μg
Unit Size:
100µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
15306934 100 μg 100µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 15306934 Supplier Invitrogen™ Supplier No. 700062

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Rabbit Recombinant Monoclonal Antibody

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. This antibody reacts with Human Vinculin. Based on sequence similarity, reactivity to chimpanzee, Rhesus monkey, swine, equine, mouse, rat, bovine, and chicken is expected. Immunogen sequence IRGALAEARKIAELCDDPKERDDILRSLGEI SALTSKLADLRRQGKGDSPEARALAKQV ATALQNLQTKTNRAVANSRPAKAAVHLE GKIEQAQRWIDNPTVDDRGVGQAAIRGL VAEGHRLANVMMGPYRQDLLAKCDRVD QLTAQLADLAARGEGESPQARALASQLQ DSLKDLKARMQEAM. Test material for western blotting/immunofluorescence: HeLa cells. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Vinculin
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Multiplex Immunohistochemistry
Classification Recombinant Monoclonal
Clone 42H89L44
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide
Gene VCL
Gene Accession No. P18206, Q64727
Gene Alias 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
Gene Symbols VCL
Host Species Rabbit
Immunogen A recombinant protein corresponding to amino acids 408-591 of P18206.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 22330, 7414
Target Species Human, Mouse
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.