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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1

Thermo Scientific™ Acclaim™ 120 C8 HPLC Columns

Achieve exceptional performance and maximum resolution for reversed-phase separation of analytes with moderate hydrophobicity.

Pore Size 120 Å
Carbon Load 11%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L7
2
Promotions Available

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3
Promotions Available

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
4
Promotions Available

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
5

Thermo Scientific™ POROS™ A 20 μm Protein A Affinity HPLC Columns

Thermo Scientific™ POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies. The 20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

EDGE
Particle Size 20 μm
Stationary Phase Recombinant Protein A
Binding Capacity 19 mg/mL
Product Line POROS
6
Promotions Available

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

EDGE
Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
7
Promotions Available

Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC Columns

Obtain exceptional peak shape and high resolution for fast UHPLC high-pressure, high-throughput applications using generic gradients on Vanquish UHPLC and LC/MS systems.

EDGE
Pore Size 175 Å
Stationary Phase C18 Selectivity
Temperature 60°C
Phase Reversed Phase
For Use With UHPLC
Endcapped Yes
Surface Area 220 m²/g
Column Format Analytical Column
Particle Size 1.9 μm
Carbon Load 10%
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 1 to 11
Product Line Hypersil GOLD
USP Type L1
8
Promotions Available

Thermo Scientific™ Acclaim™ 120 C18 Columns

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

EDGE
Pore Size 120 Å
Carbon Load 18%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L1
9
Promotions Available

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SCX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and biosimilar proteins.

EDGE
Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Proprietary
Temperature Ambient to 60°C
Phase Strong Cation Exchange
Packing Material Monodisperse Polymer Resin
Column Type Ion Exchange
Mobile Phase Compatibility Recommended for use with CX-1 pH buffers
pH 2 to 12
Technique Bio-LC
Max. Pressure 4500 psi (310 bar)
Product Line ProPac
Column Format Analytical Column
10
Promotions Available

Thermo Scientific™ Hypersil™ ODS-2 C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes separated under difficult operating conditions.

EDGE
Pore Size 80 Å
Carbon Load 11%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous Type A Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 220 m²/g
Max. Pressure 5800 psi (400 bar)
Product Line Hypersil Classical
USP Type L1
11
Promotions Available

Thermo Scientific™ EASY-Spray™ HPLC Columns
BIOPHARMA

Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.

EDGE
Phase Reversed Phase
12
Promotions Available

Thermo Scientific™ Accucore™ C18 HPLC Columns
BIOPHARMA

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

EDGE
Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 9%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 130 m²/g
Particle Shape Solid Core
USP Type L1
13
Promotions Available

Thermo Scientific™ Hypersil™ APS-2 HPLC Columns, 5μm Particle Size
BIOPHARMA

Analyze sugars with this versatile amino propyl phase of Thermo ScientificTM HypersilTM APS-2 HPLC Columns.

EDGE
Pore Size 120 Å
Carbon Load 1.9%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Packing Material Spherical, Fully Porous Type A Silica
Column Type Carbohydrate Analysis
pH 2.5 to 8
Surface Area 170 m²/g
USP Type L8
14
Promotions Available

Thermo Scientific™ Acclaim™ Trinity P2 HPLC Columns

Use these mixed-mode columns for separation of multivalent cations or anions and pharmaceuticals.

EDGE
Pore Size 300 Å
Solvents Compatible with most common HPLC organic solvents except for alcohols
Stationary Phase Spherical, Nanopolymer Hybrid Silica
Temperature 45°C
Phase HILIC and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
Green Features FSC
Surface Area 100 m²/g
Max. Pressure 6000 psi (415 bar)
Particle Size 3 μm
Mobile Phase Compatibility 0 to 100% aqueous mobile phase
pH 2.5 to 7
Particle Shape Spherical silica
Product Line Acclaim
15
Promotions Available

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
BIOPHARMA

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

EDGE