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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 3,926 results
1
Promotions Available

Thermo Scientific™ Acclaim™ 120 C18 Columns

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

EDGE
Pore Size 120 Å
Carbon Load 18%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L1
2
Promotions Available

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3
Promotions Available

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
BIOPHARMA

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

EDGE
4
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Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

EDGE
Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
5
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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
6

Thermo Scientific™ Hypersil GOLD™ Peptide HPLC Column

Hypersil GOLD™ Peptide performance is assured by our dual quality confirmation, which is performed on both neutrals compounds as well as on a Cytochrome C digest.

EDGE
Pore Size 175 Å
Temperature 60°C
Phase Reversed Phase
For Use With UHPLC
Endcapped Yes
Quantity Each
For Use With (Application) Peptide
Surface Area 220 m²/g
Carbon Load 11%
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 2 to 11
Particle Shape Fully Porous
Product Line Hypersil GOLD
USP Type L1
7
Promotions Available

Thermo Scientific™ Hypersil™ APS-2 HPLC Columns, 5μm Particle Size
BIOPHARMA

Analyze sugars with this versatile amino propyl phase of Thermo ScientificTM HypersilTM APS-2 HPLC Columns.

EDGE
Pore Size 120 Å
Carbon Load 1.9%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Packing Material Spherical, Fully Porous Type A Silica
Column Type Carbohydrate Analysis
pH 2.5 to 8
Surface Area 170 m²/g
USP Type L8
8

Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped HPLC Columns

Use this column, with its specially designed solid core, for the separation of compounds in highly aqueous mobile phases.

EDGE
Pore Size 80 Å
Stationary Phase aQ C18
Temperature 70°C
Phase Reversed Phase
Endcapped Polar
Surface Area 130 m²/g
Max. Pressure 14500 psi (1000 bar)
Particle Size 2.6 μm
Carbon Load 9%
Packing Material Ultrapure Silica
pH 2 to 9
Particle Shape Solid Core
Product Line Accucore
USP Type L1
9
Promotions Available

Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

EDGE
Shipping Condition Wet Ice
Pore Size 2000 Å
Stationary Phase Human Serum Albumin affinity ligand, humanspecific
Column Type Affinity
Quantity 1 Column
Material PEEK
High-throughput Compatibility Not High-throughput Compatible (Manual)
Max. Pressure 180 bar
Particle Size 20 μm
Flow Rate Up to 5000 cm/hr.
Matrix Polystyrenedivinylbenzene
Reactivity Human
Target Molecule HSA, HSA Fusion Proteins
Product Type Column
Binding Capacity 2.6 mg/mL
Sample Type Cell Culture Supernatants
Purification Target HSA, HSA Fusion Proteins
Product Line CaptureSelect, POROS
10
Promotions Available

Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

EDGE
Pore Size 130 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L7
11
Promotions Available

Thermo Scientific™ Hypersil GOLD™ Phenyl HPLC Columns

Use for quick analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

EDGE
Pore Size 175 Å
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L11
12
Promotions Available

Thermo Scientific™ POROS™ CaptureSelect™ CH1-XL Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ CH1-XL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands.

EDGE
Shipping Condition Wet Ice
Pore Size 2000 Å
Stationary Phase IgG Fab affinity ligand, CH1 domain of human IgG antibodies
Temperature 2-40°C
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 2400 psi (170 bar)
Column Format Analytical Column, Biocompatible PEEK
Particle Size 20 μm
Packing Material Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Binding Capacity 26.2 mg/ml human IgG 8.8 mg/ml human Fab fragment
pH 1-10
Technique Bio-LC
Product Line POROS CaptureSelect
13
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Thermo Scientific™ Acclaim™ Trinity P2 HPLC Columns

Use these mixed-mode columns for separation of multivalent cations or anions and pharmaceuticals.

EDGE
Pore Size 300 Å
Solvents Compatible with most common HPLC organic solvents except for alcohols
Stationary Phase Spherical, Nanopolymer Hybrid Silica
Temperature 45°C
Phase HILIC and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
Green Features FSC
Surface Area 100 m²/g
Max. Pressure 6000 psi (415 bar)
Particle Size 3 μm
Mobile Phase Compatibility 0 to 100% aqueous mobile phase
pH 2.5 to 7
Particle Shape Spherical silica
Product Line Acclaim
14
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Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Columns, 1.5μm
BIOPHARMA

Use the 1.5μm solid core particles of these columns with the Vanquish UHPLC system for unique high-efficiency, high-resolution analyses.

EDGE
Pore Size 80 Å
Particle Size 1.5 μm
Carbon Load 6.5%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
Diameter (Metric) 2.1 mm
pH 2 to 9
Surface Area 110 m²/g
USP Type L1
15
Promotions Available

Thermo Scientific™ DNAPac™ RP Columns
BIOPHARMA

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

EDGE