PCR Equipment and Supplies
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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25 X |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Reverse Transcriptase | ArrayScript™ |
| Concentration | 25X |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Tube |
| Product Type | One-Step RT-PCR Reagent |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Sample Type | RNA |
| Reaction Speed | Standard |
| Product Line | AgPath-ID, Ambion |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 25 X |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Reverse Transcriptase | ArrayScript™ |
| Concentration | 25X |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Tube |
| Product Type | One-Step RT-PCR Reagent |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 System |
| Reaction Speed | Standard |
| Product Line | AgPath-ID, Ambion |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
| Polymerase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Standard |
| Hot Start | No |
| Overhang | 3'-A |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Standard PCR |
DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | DreamTaq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Tissue |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Format | Kit |
| Sample Type | PCR Products |
| Isolation Technology | Silica Spin Column |
| Run Time | 10 min. |
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 8 kb |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Universal Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Fisherbrand™ PCR™ Cooler, 96-Well, 2 Pack
The PCR™ Cooler is ideal for thermosensitive applications, PCR, or cell-based assays, as well as gentle sample thawing and stopping of reactions. The unit also provides a dependable cooling station for pipetting and sample storage. Perfect for transporting samples and reactions between preparation, thermal cycling and post-PCR procedure areas.
| Height (Metric) | 1.9 cm |
|---|---|
| Content And Storage | Lid, Rack |
| Description | Fisherbrand™ PCR™ Cooler, 96-Well, 2 Pack |
| Capacity | Tubes |
| For Use With (Equipment) | Shakers, Mixers, Vortexers |
| Depth (Metric) | 4.8 cm |
| Quantity | 2 |
| Vessel Type | PCR Rack |
| Thermal Accuracy | ±1°C |
| For Use With (Application) | PCR Applications |
| Temperature Range (Metric) | 0°C to 5°C |
| Width (Metric) | 14.8 cm |
| Weight | .27 kg (0.6 lb.) |