Protein Purification Reagents and Kits
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Filtered Search Results
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Human) Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Hu) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the Fc part (CH3 domain) of all four human IgG subclasses.
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Target | IgG (Fc region) |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Thermo Scientific™ Pierce™ Glutathione Agarose
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
| Stationary Phase | Glutathione |
|---|---|
| Type | Agarose |
| Content And Storage | Upon receipt store at 4°C. |
| Format | Bottle |
| Protein Form | Recombinant |
| Column Type | Affinity, Agarose Resin |
| Capacity (Metric) | 40 mg Purified Recombinant GST Protein Binding per mL Resin |
| Product Line | Pierce |
Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix
Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix.
| Particle Size | 65 μm |
|---|---|
| Ligand Type | CH1-XL affinity ligand |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Binding Capacity | >15 mg/mL |
| Form | Agarose Bead |
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
| Stationary Phase | Proprietary Ligand |
|---|---|
| Column Type | Proprietary Resin, Affinity |
| Purification Target | Detergent Removal |
Thermo Scientific™ Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin
Pierce High-Capacity EDTA-Compatible Ni-IMAC Resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.
| Binding Property | Chelator Stable |
|---|
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa recombinant single domain antibody fragment that specifically binds to the Fc part of IgG from multiple species.
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Target | IgG (Fc region) |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin
Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.
| Shipping Condition | Wet Ice |
|---|---|
| Stationary Phase | Anti-DYKDDDDK monoclonal antibodies |
| Product Type | Affinity Resin |
Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate
Thermo Scientific™ CaptureSelect™ Biotin Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid - proline - glutamic acid - alanine).
| Ligand Type | Antibody |
|---|---|
| Type | Conjugate |
| Content And Storage | Store at -5°C to -30°C. |
| Form | Liquid |
| Label or Dye | Biotin & Other Haptens |
| Product Line | CaptureSelect |
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
| Stationary Phase | Divalent Nickel, Metal Chelate |
|---|---|
| Type | Resin |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Description | HisPur Ni-NTA Resin |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
| Product Line | HisPur |
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
| Buffer | Lysis Buffer |
|---|---|
| Shipping Condition | Ambient |
| Form | Liquid |
| Product Type | Nuclear and Cytoplasmic Extraction Reagent |
| Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
| Product Line | NE-PER |