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Cytiva PuReTaq Ready-To-Go™ PCR Beads
Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.
| Quantity | 1 Kit |
|---|---|
| For Use With (Application) | Standard PCR |
Cytiva NAP™ Columns, NAP-10
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
| Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
|---|---|
| Product Type | NAP-10 |
| For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva NAP™ Columns, NAP-5
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
| Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
|---|---|
| Product Type | NAP-5 |
| For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva ExoProStar™ 1-step
Use for enzymatic PCR clean-up. Cytiva Lifescience™ Illustra ExoProStar™ 1-step with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs.
| Description | Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube. |
|---|---|
| Product Type | illustra ExoProStar 1-Step |
| Isolation Technology | Enzymatic PCR Clean-up Technology |
| Quantity | 1 Each |
| Yield | 100% recovery of PCR product |
| For Use With (Application) | Remove unincorporated primers and dNTPs |
Cytiva GFX™ PCR DNA and Gel Band Purification Kit
Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. Cytiva Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100μL or agarose gel slices of up to 900mg.
| Includes | GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers |
|---|---|
| Description | Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. |
| Product Type | illustra GFX PCR DNA and Gel Band Purification Kit |
| Formulation | Tris HCl and sterile Water |
| Sample Type | Agarose Gel Slices,DNA Solutions,Enzyme Reactions,PCR Mixtures |
| Sample Volume | 50 bp-10 kb |
| Yield | PCR 10 μl elution volume-65%, PCR 50 μl elution volume-82%, 300 mg agarose 10 μl elution volume-57%, 300 mg agarose 50 μl elution volume-91% |
| For Use With (Application) | Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions |
Cytiva Amersham™ ECL™ Blocking Agent
Use for blocking nitrocellulose and PVDF membranes. Cytiva Life Sciences™ Amersham™ ECL™ Blocking Agent blocks membranes to be used with ECL and ECL Plus™ Western blotting detection reagents.
Cytiva MicroSpin™ S-400 HR Columns
Use for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides. Cytiva Life Sciences™ illustra™ MicroSpin™ S-400 HR Columns use spin-column chromatography for quick results.
Cytiva Ready-to-Go™ You-Prime First-Strand Beads
Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. Cytiva Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.
Cytiva TempliPhi™ 100/500 Amplification Kit
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
| Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |
|---|---|
| Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification |
| Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
| For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
| Test Time | 4-18 h depending on starting material |
Cytiva GenomiPhi V2 DNA Amplification Kit
Amplify whole genomes with high efficiency and representation. Cytiva Life Sciences™ illustra™ GenomiPhi V2 DNA Amplification Kit provides whole genome amplification with yields of 4 to 7μg.
| Content And Storage | Store the kit at -70°C.The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. Thaw components on ice and maintain at 0°C to 4°C during handling. |
|---|---|
| Description | Illustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample. |
| Product Type | illustra GenomiPhi V2 DNA Amplification Kit |
| Size | > 10 kb |
| For Use With (Application) | Genomic DNA Amplification |
| Test Time | 1.5-2 h |
Cytiva Microarray Hybridization Solution v2.0
Uses a blend of proprietary components to provide quality automated and manual slide hybridizations. Cytiva Microarray Hybridization Solution v2.0 features hybridization rate enhancers that improve the detection of rare targets, plus blocking agents that minimize nonspecific background.
Cytiva ProbeQuant™ G-50 Micro Columns
For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50μL. Cytiva Life Sciences™ ProbeQuant™ G-50 Micro Columns use spin-column chromatography.
Cytiva blood genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow and nucleated red blood cells.
| Includes | Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card |
|---|---|
| Description | illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells. |
| Product Type | illustra blood genomicPrep Mini Spin Kit |
| Purity | 1.7 |
| Sample Volume | 50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡ |
| Yield | 4-6 μg/200 μl whole blood |
| For Use With (Application) | Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest |
| Test Time | < 20 min |
Cytiva Amersham™ Streptavidin-HRP Conjugate
Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization applications
| Includes | 2mL of Phosphate Buffered Saline (0.1M Sodium Phosphate, 0.1M Sodium Chloride, pH 7.4) containing 1%(w/v) Bovine Serum Albumin and an antimicrobial agent |
|---|---|
| For Use With (Application) | Suitable for use in ECL™, western blotting ,ELISA, immunocytochemistry |
Cytiva Nucleon™ PhytoPure™ Genomic DNA Extraction Kits
Nucleon PhytoPure DNA Extraction kits use a phytopure resin to bind plant polysaccharides ensuring high yields of high-quality plant genomic DNA in only one hour
| Includes | Reagents 1 and 2 and PhytoPure™ Resin |
|---|---|
| Description | Quality, length, and purity of a phenol-chloroform extraction without phenol. Nucleon PhytoPure enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well |
| Sample Type | Barley (Fresh, Frozen or Freeze Dried),Maize (Fresh, Frozen or Freeze Dried),Rye (Fresh, Frozen or Freeze Dried),Wheat (Fresh, Frozen or Freeze Dried) |
| For Use With (Application) | Successfully extracts DNA from many plant species, starting with fresh, frozen, or freeze dried material |