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Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
| Product Type | T4 DNA Ligase |
|---|---|
| Enzyme | DNA Ligase |
| Compatible Buffer | 10X T4 DNA Ligase Buffer |
| Concentration | 5 U/μL |
Thermo Scientific™ Eco32I (EcoRV) (10 U/μL)
The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV).
| Concentration | 10 U/μL |
|---|
Invitrogen™ RNase Cocktail™ Enzyme Mix
Endonuclease-free, exonuclease-free, protease-free
| Shipping Condition | Dry Ice |
|---|---|
| Product Type | RNAse Cocktail Enzyme Mix |
| Enzyme | RNase |
| Product Line | Ambion™ |
Thermo Scientific™ NTP Set, 100 mM Solution
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
| Polymerase | DNA Polymerase I |
|---|---|
| Product Type | Klenow Fragment |
| Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
| Concentration | 5 U/μL |
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
| Product Type | T4 DNA Ligase |
|---|---|
| Enzyme | Ligase |
| Concentration | 1 U/μL |
Invitrogen™ ezDNase™ Enzyme
SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
Thermo Scientific™ NdeI (10 U/μL)
The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI).
| Concentration | 10 U/μL |
|---|
Invitrogen™ T4 DNA Ligase (5 U/μL)
Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini
Thermo Scientific™ BglII (10 U/μL)
Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
| Product Type | TdT |
|---|---|
| Enzyme | Terminal Deoxynucleotidyl Transferase |
| Compatible Buffer | Storage Buffer, 5X Reaction Buffer |
| Concentration | 20 U/μL |
Invitrogen™ M13KO7 Helper Phage
Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication
Thermo Scientific™ XmaJI (AvrII) (10 U/μL)
Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XmaJI (AvrII) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |