Protein Analysis Reagents
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Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
| MWCO | 7.0 kDa |
|---|---|
| Column Type | Size-exclusion, Proprietary Resin |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Zeba |
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Thermo Scientific™ Pierce™ C18 Spin Tips & Columns
Thermo Scientific Pierce C18 Spin Columns (10–150 μL) and Spin Tips (10–100 μL) effectively remove interfering contaminants, while also maintaining excellent binding and recovery of peptide samples for use upstream of mass spectrometry and other high sensitivity detection methods.
| Content And Storage | Store at room temperature. |
|---|---|
| Workflow Step | Sample Clean Up |
| Final Product Type | Peptides |
| For Use With (Equipment) | Microcentrifuge, Mass Spectrometer |
| Starting Material | Peptides,Protease-digested Protein |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein G covalently bound to Dynabeads |
| Surface Functionality | Protein G |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein G |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays.
| Product Line | Qubit |
|---|---|
| Detection Method | Fluorescence |
Thermo Scientific™ Pierce™ HeLa Protein Digest Standard
Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
| Form | Solid |
|---|---|
| Final Product Type | Peptides |
| Product Type | Protein Digest Standard |
| For Use With (Equipment) | Mass Spectrometer |
| Reagent Type | Standard |
| Starting Material | Peptides |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO
Remove salts and small contaminants to maximize sample purity and recovery with Slide-A-Lyzer 10K MWCO dialysis cassettes.
| MWCO | 10 kDa |
|---|---|
| Content And Storage | Store at room temperature |
| Format | Cassette |
| Membrane | Cellulose Membrane, Dialysis |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Slide-A-Lyzer |
Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown
Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.
| Content And Storage | Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C. |
|---|---|
| Final Product Type | His-Tagged Protein |
| Color | Brown |
| Binding Property | 1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads |
| Material | Magnetic Polystyrene |
| Concentration | 40 mg/mL |
| For Use With (Application) | Isolation of His-tagged Recombinant Proteins |
| High-throughput Compatibility | Yes |
| Regulatory Status | For Research Use Only |
| Ligand Type | Nitrilotriacetic acid (NTA) |
| Type | Magnetic Beads |
| Target | His-tagged proteins |
| Format | Magnetic beads |
| For Use With (Equipment) | KingFisher Sample Purification System, DynaMag magnets |
| Sample Type | Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts |
| Diameter (Metric) | 1 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Shelf Life | 36 months from date of manufacture |
| Product Line | Dynabeads |
Thermo Scientific™ Pierce™ High Sensitivity Streptavidin-HRP
Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.
| Content And Storage | Storage at 4°C for at least one year. |
|---|---|
| Conjugate | HRP |
| Target Molecule | Biotin |
| Concentration | 1.0–1.1 mg/mL |
| Product Line | Pierce |
Gibco™ FreeStyle™ CHO Expression Medium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Animal origin-free, chemically defined, protein-free medium
| Cell Line | FreeStyle™ CHO-S Cells, CHO |
|---|---|
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Form | Liquid |
| Product Type | Expression Medium |
| Endotoxin Level | ≤0.25 EU/mL, ≤ 0.25 EU/mL |
| Classification | Chemically Defined, Protein-Free, Serum-Free |
| Culture Type | Suspension Cell Culture |
| Phenol Red Indicator | No Phenol Red |
| Product Line | Freestyle, Gibco |
| Serum Level | Serum-Free |
| Glutamine | No Glutamine |
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor, Phosphatase Inhibitor |
| Form | Liquid |
| Product Type | Protease and Phosphatase Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor, Phosphatase Inhibitor |
| Product Line | Halt |