CD178 Mouse anti-Human, Biotin, Clone: NOK-1, BD

Description

Fas (CD95; APO-1) is a 45kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or by Fas ligand (FasL; CD178). Fas belongs to the TNF (Tumor Necrosis Factor)/NGF (Nerve Growth Factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. CD178 (FasL), a member of the TNF cytokine family, induces apoptosis by binding to Fas, its cell-surface receptor. FasL may exist as either membrane bound or soluble forms and is expressed by activated T and NK cells. FasL may also be constitutively expressed in some immunologically privileged sites, e.g., eye and testis. Fas and FasL play an important role in the induction of apoptosis, and thus regulate a variety of immunological responses. The NOK-1 antibody clone has been reported to recognize human FasL, recognizing both the membrane bound (FasL) and soluble (sFasL) forms. FasL and sFasL have been reported to migrate at reduced molecular weights of 40 and 26kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in the literature. The NOK-1 antibody clone is not
recommended for the western blot application.

Host Species: Mouse
Clone: NOK-1
Isotype: IgG1
Species Reactivity: Human
Immunogen: Mouse T lymphoma cells (L5178Y) expressing human FasL

Flow Cytometry