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Cytochrome c Antibody (7H8.2C12) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 54 publications

208.00 € - 587.00 €

Specifications

Antigen Cytochrome c
Clone 7H8.2C12
Concentration 1.0 mg/mL
Dilution Western Blot 0.05-0.5 ug/ml, Simple Western 2.5 ug/ml, Flow Cytometry 1:20-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006)), Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Fujimara et al (1998)), Immunoblotting reported in scientific literature (PMID 16436379), Flow (Cell Surface) reported in scientific literature (PMID 16467206), Flow (Intracellular) reported in scientific literature (Mohr et al (2004))
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
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Product Code Brand Quantity Price Quantity & Availability  
18749433
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Novus Biologicals
NB100-56503SS
0.025 mg
208.00 €
25µg
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18727403
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Novus Biologicals
NB100-56503
0.1 mg
587.00 €
0.10mg
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Description

Description

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Mouse, Rat, Drosophila, Rabbit samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Flow (Cell Surface), Flow (Intracellular).
Specifications

Specifications

Cytochrome c
1.0 mg/mL
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Unconjugated
Mouse
Apoptosis, Cellular Markers, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
P99999
54205
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
7H8.2C12
Western Blot 0.05-0.5 ug/ml, Simple Western 2.5 ug/ml, Flow Cytometry 1:20-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006)), Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Fujimara et al (1998)), Immunoblotting reported in scientific literature (PMID 16436379), Flow (Cell Surface) reported in scientific literature (PMID 16467206), Flow (Intracellular) reported in scientific literature (Mohr et al (2004))
Monoclonal
Purified
RUO
Human, Mouse, Rat, Drosophila, Rabbit
CYCHCS, cytochrome c, cytochrome c, somatic, THC4
CYCS
IgG2b κ
Protein G purified
This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
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