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GAPDH Antibody (13H12) - Azide and BSA Free, Novus Biologicals™

Product Code. 18281788 Shop All Bio Techne Products
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Product Code. 18281788 Supplier Novus Biologicals Supplier No. NBP280749

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Description

Mouse Monoclonal Antibody

GAPDH Monoclonal specifically detects GAPDH in Human, Mouse, Rat, Drosophila, Primate, Monkey, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunocytochemistry.
TRUSTED_SUSTAINABILITY

Specifications

Antigen GAPDH
Applications Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone 13H12
Concentration 0.5 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25 - 1 ug/ml. Use reported by customer review, Simple Western : 1:25, Immunohistochemistry 5 ug/ml, Immunocytochemistry/Immunofluorescence : 1:10, Immunohistochemistry-Paraffin : 5 ug/ml, Immunocytochemistry
Formulation PBS
Gene Alias aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
Gene Symbols GAPDH
Host Species Mouse
Immunogen Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this GAPDH antibody.
Purification Method Protein G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Alzheimers Research, Apoptosis, Autophagy, Cancer, Chromatin Research, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
Primary or Secondary Primary
Gene ID (Entrez) 2597
Test Specificity Based upon 91% sequence similarity with immunogen, this antibody is predicted to react with Guinea Pig, Sheep, Squirrel, Porcine/Pig, Ferret, Canine/Dog/Cat, Bovine, Reptile/ Rattlesnake and several other species. Immunogen shows 82% similarity to Xenopus and Zebrafish. Rat, sheep, and monkey reactivity reported in scientific literature (PMID: 24796753, PMID: 27618403, and PMID: 24462973 respectively).
Target Species Human, Mouse, Rat, Drosophila, Primate, Monkey, Sheep
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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