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TNF-alpha Antibody - Azide Free, Novus Biologicals™

Product Code. 18705173 Shop All Bio Techne Products
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Product Code. 18705173 Supplier Novus Biologicals Supplier No. NB600587SS

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Description

Rabbit Polyclonal Antibody has been used in 18 publications

TNF-alpha Polyclonal specifically detects TNF-alpha in Human, Mouse, Rat, Chicken, Primate samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TNF-alpha
Applications Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration 75 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:500-1:2000, ELISA 1:1000-1:5000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100-1:500
Formulation Antiserum with No Preservative
Gene Accession No. P01375, P01375, P01375, P01375
Gene Alias APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene Symbols TNF
Host Species Rabbit
Immunogen The whole rabbit serum was prepared by repeated immunizations with recombinant human TNF-alpha produced in E.coli. (Uniprot: P01375)
Molecular Weight of Antigen 25.64 kDa
Purification Method Whole Antiserum
Quantity 0.04 mL
Regulatory Status RUO
Research Discipline Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Primary or Secondary Primary
Gene ID (Entrez) 7124
Test Specificity The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Target Species Human, Mouse, Rat, Primate
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Form Antisera
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