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TNF-alpha Antibody - Azide Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 18 publications

183.00 € - 415.00 €

Specifications

Antigen TNF-alpha
Concentration 75 mg/mL
Dilution Western Blot 1:500-1:2000, ELISA 1:1000-1:5000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100-1:500
Applications Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Classification Polyclonal
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Product Code Brand Quantity Price Quantity & Availability  
18705173
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Novus Biologicals
NB600-587SS
0.04 mL
183.00 €
0.04mL
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18769593
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Novus Biologicals
NB600-587
0.2 mL
415.00 €
0.20mL
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Description

Description

TNF-alpha Polyclonal specifically detects TNF-alpha in Human, Mouse, Rat, Chicken, Primate samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
Specifications

Specifications

TNF-alpha
Western Blot 1:500-1:2000, ELISA 1:1000-1:5000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100-1:500
Polyclonal
Antisera
RUO
Human, Mouse, Rat, Primate
P01375, P01375, P01375, P01375
7124
The whole rabbit serum was prepared by repeated immunizations with recombinant human TNF-alpha produced in E.coli. (Uniprot: P01375)
Whole Antiserum
The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
75 mg/mL
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Unconjugated
Rabbit
Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Antiserum with No Preservative
APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
TNF
Primary
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
25.64 kDa
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