Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°C to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | DPBS |
| Recommended Storage | 15°to 30°C |
| Concentration | 1 X |
Gibco™ PBS (10X), pH 7.4
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
| Includes | No Sodium Pyruvate |
|---|---|
| Color | White |
| Physical Form | Liquid |
| pH | 7.4 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Storage conditions: 15°C to 30°C, Shipping conditions: Room temperature, Shelf life: 24 months from date of manufacture |
| Concentration | 10X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2800 to 3100 mOsm/kg |
| Sterility | Sterile |
| Solution Type | pH Buffer, Storage Solution and Cleaning Solution |
| Product Line | Gibco |
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| pH | 7.4 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Ambient temperatures |
| Concentration | 10 X |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
| Physical Form | Liquid |
|---|---|
| pH | 7.1 to 7.3 |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°to 30°C |
| Concentration | 10 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 2800 to 3200 mOsm/kg |
Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Corning™ Cell Culture Phosphate Buffered Saline (1X)
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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With low endotoxin content. Without calcium and magnesium.
| Formulation | Potassium Dihydrogen Phosphate 0.144g/L, Sodium Chloride 9g/L, Disodium Phosphate 0.795g/L |
|---|---|
| Physical Form | Liquid |
| pH | 7.3 to 7.5 |
| Recommended Storage | 2° to 30°C |
| Concentration | 1 X |
Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
Supports traceability with guaranteed access to certificates and proactive change notifications.
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With low endotoxin content
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Room temperature |
Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)
Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels.
MP Biomedicals™ Phosphate Buffered Saline Tablets (PBS)
Phosphate Buffered Saline Tablets (PBS) are a balanced salt mixture for easy PBS buffer preparation for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and cell culture reagent.
| Includes | No Calcium, No Magnesium |
|---|---|
| Molecular Weight (g/mol) | 74.55 |
| MDL Number | MFCD00011360 |
| Physical Form | Tablet |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| SMILES | [Cl-].[K+] |
| IUPAC Name | potassium chloride |
| InChI Key | WCUXLLCKKVVCTQ-UHFFFAOYSA-M |
| Molecular Formula | ClK |
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-EDTA |
| Grade | Molecular Biology |
| DNase | DNase free |
| Filtered Through | Filtered through a 5-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 1X Solution, pH 8.0 |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Health Hazard 2 | CAUTION! |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| Recommended Storage | RT |