Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.

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Invitrogen™ mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit

The mMESSAGE mMACHINE T7 ULTRA Transcription Kit generates RNA transcripts with higher protein yields due to the combination of the anti-reverse cap analog (ARCA) with patented transcription reagents.

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Invitrogen™ MEGAscript™ SP6 Transcription Kit

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

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BD Pharmingen™ Transcription-Factor Buffer Set

Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins

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Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

in vitro synthesis of large amounts of capped RNA

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Invitrogen™ MEGAscript™ T7 Transcription Kit

Ultra-high yield technology for in vitro transcription.

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Invitrogen™ mMESSAGE mMACHINE™ T7 mRNA Kit with CleanCap™ Reagent AG

The mMESSAGE mMACHINE T7 mRNA Kit with CleanCap Reagent AG contains all the reagents needed to generate mRNA with naturally occurring Cap 1 structure while providing increased yields and higher quality with greater translatability in vivo compared to legacy capping methods such as ARCA caps.

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Specifications

Format	Kit
Isolation Technology	Silica Spin Column

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Invitrogen™ MEGAclear™ Transcription Clean-Up Kit

The MEGAclear kit can be used to remove nucleotides, short oligonucleotides, proteins, and salts from RNA. The RNA recovered can be used for any application that requires high purity RNA. Recover from 1 ng to 500 μg of RNA.

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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions.

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Invitrogen™ MEGAshortscript™ T7 Transcription Kit

Maximize the yield of short RNA transcripts

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Invitrogen™ CytoTune™-iPS 2.0 Sendai Reprogramming Kit

The CytoTune-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune-iPS Sendai Reprogramming Kit.

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Specifications

Shipping Condition	Dry Ice
Promoter	Proprietary
Format	Kit
Sub Cellular Localization	Cytoplasm
Product Type	Sendai Reprogramming Kit
Delivery Type	Sendai virus
For Use With (Application)	Stem Cell Engineering & Reprogramming
Product Line	CytoTune™
Cell Type	iPSC

Invitrogen™ Cap Analog (m⁷G(5')ppp(5')G), 10 A₂₅₄ units

For 5' capped RNA molecules synthesis as part of in vitro transcription reactions

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Invitrogen™ MAXIscript™ T7 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

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Specifications

Promoter	T7
Content And Storage	T7 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 μg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

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Invitrogen™ mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit

Generate RNA transcripts with higher protein yields

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Invitrogen™ MAXIscript™ SP6 Kit with Manual

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 μg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

Invitrogen™ MAXIscript™ SP6/T7 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Promoter	T7, SP6
Content And Storage	SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Product Type	SP6/T7 In Vitro Transcription kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 μg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

Transcription and Translation Reagents

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