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DNA Extraction and Purification

Kits, reagents, and other supplies for the extraction of DNA from a sample, and its subsequent purification. Products include plasmid miniprep and maxiprep kits, plasmid purification kits, PCR purification kits, and genomic DNA purification kits.
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1 – 15 of 64 results
1

Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane.

EDGE
Shipping Condition Room Temperature
Content And Storage • Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes
Isolation Technology Anion Exchange Resin
Scale Maxi
High-throughput Compatibility Manual Protocols
For Use With (Application) Cloning, PCR
Expression System Mammalian
Test Time 90 min.
Prep Scale > 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA
Target Plasmid DNA
Format Spin Column
Product Type Plasmid Purification Kit
Sample Type Bacteria
Product Line PureLink™
2

Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit

The PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin.

EDGE
3

Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit

The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times.

EDGE
4

Invitrogen™ PureLink™ HiPure Expi Plasmid Gigaprep Kit

The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times.

EDGE
5
Promotions Available

Thermo Scientific™ GeneJET Plasmid Miniprep Kit

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

Target Plasmid DNA
Format Spin Column
Product Type Plasmid MiniPrep Kit
Sample Type Bacteria
Isolation Technology Silica Column
Column Type Spin Column
Scale Mini
High-throughput Compatibility Compatible (Vacuum manifold)
For Use With (Application) PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
Test Time <14 min
Product Line GeneJET
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
6

Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.

EDGE
Shipping Condition Room Temperature
Content And Storage • Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature
Format Kit
Product Type Plasmid Purification Kit
Isolation Technology Silica Column
Scale Maxi
For Use With (Application) Cloning, PCR
Test Time 30 min.
Product Line PureLink™
Prep Scale Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg
7

Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit

The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.

EDGE
Content And Storage The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature.
Final Product Type Plasmid DNA
Isolation Technology Anion Exchange Resin
Column Type Filter Column
System Type PureLink™
Purity Transfection Grade
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Test Time 1 hr.
Prep Scale > 200 μg (Large-Scale) Plasmid DNA
Plasmid <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Target Plasmid DNA
Format Column
Product Type Plasmid FP MaxiPrep Kit
Sample Type Bacterial Culture
Yield 1000 μg
Elution Volume 15 mL
Product Line PureLink™
8

Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit

The MagMAX Pro HT NoSpin Plasmid Miniprep Kit enables scalable, ultra-rapid purification of high-quality, transfection-grade plasmid DNA (pDNA) from bacterial cultures. Using magnetic-bead technology, it simplifies workflows by eliminating centrifugation and vacuum filtration bottlenecks.

EDGE
Content And Storage 2°C to 8°C BactBind Beads, PlasmidPure Beads, Endotoxin Reduction Buffer
15°C to 25°C RNAse A, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, Wash 1 Buffer, Wash 2 Buffer, Elution Buffer
Isolation Technology Magnetic Bead
Purity Transfection Grade
Sample Volume Bacterial Culture, 1 mL per well
Quantity 1 Ea.
Green Features Energy-efficient design, reduced packaging, and elimination of volatile organic solvents
Scale Mini
For Use With (Application) Cloning, Transfection, Protein Expression, Protein Production, mRNA production via In Vitro Transcription, Gene Editing, Sequencing, PCR, Nucleic acid labeling, PCR
Size Distribution 5-12kb
Test Time ~ 32 min. for 96 samples, 1 KingFisher 96 DWP
Plate Compatibility KingFisher 96 Deep Well Plates with or without barcodes
Format 96 Deep-well Plate
Product Type Automated, pDNA extraction kit
For Use With (Equipment) KingFisher Apex, Flex and Presto Processors with 96 Deep Well Heads, Liquid Handling Robotic Systems
Sample Type Bacteria, E. coli
Yield Up to 20 μg pDNA using high copy plasmid
Nucleic Acid Type Plasmid DNA (pDNA)
Elution Volume 100 μL
9
Promotions Available

Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.

Target Plasmid DNA
Format Spin Column
Product Type Plasmid MaxiPrep Kit
Sample Type Bacteria
Isolation Technology Silica Column
Column Type Spin Column
Scale Maxi
Concentration 400 ng/μL
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
Test Time 60 min.
Product Line GeneJET
Prep Scale > 200 μg (Large-Scale) Plasmid DNA
10
Promotions Available

Thermo Scientific™ GeneJET Gel Extraction and DNA Cleanup Micro Kit

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.

Target DNA from Enzymatic Reactions (e.g. PCR)
Format Spin Column
Product Type Gel Extraction and DNA Cleanup Kit
Sample Type DNA,Gel Samples
Isolation Technology Silica Column
Column Type Spin Column
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription
Test Time 15 min.
Product Line GeneJET
11

Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.

Target Plasmid DNA
Format Spin Column
Product Type Plasmid MidiPrep Kit
Sample Type Bacteria
Isolation Technology Silica Column
Column Type Spin Column
Scale Midi
Concentration 400 ng/μL
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
Test Time 60 min.
Product Line GeneJET
Prep Scale 100-200 μg (Medium-Scale) Plasmid DNA
12

Thermo Scientific™ MagJET Plasmid DNA Kit

Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4–5 μg/mL) or high copy plasmid DNA from overnight E. coli culture.

Target Plasmid DNA
Format Kit
Product Type Plasmid DNA Kit
Sample Type Bacteria
Isolation Technology Magnetic Bead
High-throughput Compatibility High-throughput Compatible, Not High-throughput Compatible (Manual)
For Use With (Application) Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
Product Line MagJET
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
13
Promotions Available

Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column.

14
Promotions Available

Thermo Scientific™ GeneJET Plant Genomic DNA Purification Kit

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types.

Sample Type Plants
Column Type Spin Column
High-throughput Compatibility Not High-throughput Compatible (Manual)
15
Promotions Available

Invitrogen™ DNAzol™ BD Reagent, for isolation of genomic DNA from whole blood

DNAzol BD Reagent is a ready-to-use organic reagent specifically formulated to react in about 30 minutes.