DNA Extraction and Purification
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Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit
The PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin.
Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit
PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of highest purity from 25 to 100 mL of overnight bacterial culture.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt. |
| Final Product Type | Plasmid DNA |
| Isolation Technology | Anion Exchange Resin |
| Column Type | Filter Column |
| System Type | PureLink™ |
| Purity | Transfection Grade |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| Test Time | 90 min. |
| Prep Scale | 100-200 μg (Medium-Scale) Plasmid DNA |
| Plasmid | <40kb, Low Copy Plasmid, High Copy Plasmid, BAC |
| Target | Plasmid DNA |
| Format | Column |
| Product Type | Plasmid Filter MidiPrep Kit |
| Sample Type | Bacterial Culture |
| Yield | 350 μg |
| Elution Volume | 5 mL |
| Product Line | PureLink |
Invitrogen™ PureLink™ HiPure Expi Plasmid Gigaprep Kit
The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times.
Thermo Scientific™ GeneJET Plasmid Miniprep Kit
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
| Target | Plasmid DNA |
|---|---|
| Format | Spin Column |
| Product Type | Plasmid MiniPrep Kit |
| Sample Type | Bacteria |
| Isolation Technology | Silica Column |
| Column Type | Spin Column |
| Scale | Mini |
| High-throughput Compatibility | Compatible (Vacuum manifold) |
| For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
| Test Time | <14 min |
| Product Line | GeneJET |
| Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit
The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.
| Content And Storage | The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature. |
|---|---|
| Final Product Type | Plasmid DNA |
| Isolation Technology | Anion Exchange Resin |
| Column Type | Filter Column |
| System Type | PureLink™ |
| Purity | Transfection Grade |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription |
| Test Time | 1 hr. |
| Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
| Plasmid | <40kb, Low Copy Plasmid, High Copy Plasmid, BAC |
| Target | Plasmid DNA |
| Format | Column |
| Product Type | Plasmid FP MaxiPrep Kit |
| Sample Type | Bacterial Culture |
| Yield | 1000 μg |
| Elution Volume | 15 mL |
| Product Line | PureLink |
Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
| Format | Kit |
| Product Type | Plasmid Purification Kit |
| Isolation Technology | Silica Column |
| Scale | Maxi |
| For Use With (Application) | Cloning, PCR |
| Test Time | 30 min. |
| Product Line | PureLink |
| Prep Scale | Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg |
Macherey-Nagel™ NucleoBond™ Finalizer for desalting and concentration of plasmid DNA
Concentrate and desalinate plasmid and cosmid DNA eluates obtained by anion-exchange chromatography. The NucleoBond™ Finalizer is a quick, efficient alternative to centrifugation and perfect for all types of downstream applications.
| Product Type | Desalting tool |
|---|---|
| Size | 2-50kbp |
| For Use With (Application) | Applications: Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography, Typical downstream applications: transfection, in-vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR. |
| Test Time | 5min. |
Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
| Column Type | Spin Column |
|---|---|
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.
| Target | Plasmid DNA |
|---|---|
| Format | Spin Column |
| Product Type | Plasmid MaxiPrep Kit |
| Sample Type | Bacteria |
| Isolation Technology | Silica Column |
| Column Type | Spin Column |
| Scale | Maxi |
| Concentration | 400 ng/μL |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
| Test Time | 60 min. |
| Product Line | GeneJET |
| Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
Thermo Scientific™ GeneJET Gel Extraction and DNA Cleanup Micro Kit
The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
|---|---|
| Format | Spin Column |
| Product Type | Gel Extraction and DNA Cleanup Kit |
| Sample Type | DNA,Gel Samples |
| Isolation Technology | Silica Column |
| Column Type | Spin Column |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing, PCR, In Vitro Transcription |
| Test Time | 15 min. |
| Product Line | GeneJET |
Thermo Scientific™ Silica Bead DNA Gel Extraction Kit
Perform silica bead-based DNA extraction from agarose gels and reaction mixtures with this kit, which is flexible to scale up or down both reaction and elution volumes.
Invitrogen™ DNAzol™ BD Reagent, for isolation of genomic DNA from whole blood
DNAzol BD Reagent is a ready-to-use organic reagent specifically formulated to react in about 30 minutes.
Thermo Scientific™ GeneJET Plant Genomic DNA Purification Kit
Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types.
| Sample Type | Plants |
|---|---|
| Column Type | Spin Column |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ MagJET Plasmid DNA Kit
Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4–5 μg/mL) or high copy plasmid DNA from overnight E. coli culture.
| Target | Plasmid DNA |
|---|---|
| Format | Kit |
| Product Type | Plasmid DNA Kit |
| Sample Type | Bacteria |
| Isolation Technology | Magnetic Bead |
| High-throughput Compatibility | High-throughput Compatible, Not High-throughput Compatible (Manual) |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
| Product Line | MagJET |
| Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit
Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
| Column Type | Spin Column |
|---|---|
| Scale | Mini |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |