Restriction Enzymes

1 – 15 of 46 results

Components	10X OPTIZYME™ Buffer 4
Cut Site	CCC.GGG
Form	Liquid
Product Type	SmaI
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	30°C
Concentration	10 U/μL
For Use With (Application)	>90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI

Components	10X OPTIZYME™ Buffer 4
Cut Site	T.CTAGA
Form	Liquid
Product Type	XbaI
Storage Buffer	10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme

Components	25 to 50% Water, >50% Glycerin
Cut Site	GC.GGCCGC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	NotI
Storage Buffer	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Concentration	10 U/μL
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site	A.GATCT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	BglII
Storage Buffer	10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII

Components	>50% Water, 25 to 50% Glycerin
Cut Site	AT.CGAT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	ClaI (Bsu15I)
Storage Buffer	10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL

Promotions Available

Optimize digestions with KpnI restriction enzyme using this buffer, premixed with BSA for enhanced stability.

Promotions Available

Optimize digestions with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes using this buffer.

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site	G.GATCC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	BamHI
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI

Components	25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
Cut Site	G.AATTC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	EcoRI
Storage Buffer	10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI