DNA Extraction and Purification
The isolation of DNA from different sample types and its subsequent purification requires a range of reagents and consumables. We offer a comprehensive portfolio of kits and chemicals for plasmid (miniprep, midiprep, maxiprep), PCR, and genomic DNA purification, as well as gel extraction and more.
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Product Code 10054394
Cytiva NAP™ Columns, NAP-5
Product Code 11941411
Cytiva ExoProStar™ 1-step
Product Code 11452420
MP Biomedicals™ Lysing Matrix E, 2 mL Tubes
Product Code 10004064
Cytiva NAP™ Columns, NAP-25
Product Code 11551645
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Product Code 11452410
MP Biomedicals™ Lysing Matrix B, 2 mL Tubes
Product Code 10218134
Cytiva GFX™ PCR DNA and Gel Band Purification Kit
Product Code 10591758
MP Biomedicals™ Lysing Matrix A, 2 mL Tubes
Product Code 11783299
Cytiva Microspin Column
Product Code 30067474
MP BIOMEDICALS MagBeads FastDNA Kit Ready-to-Use for MPure-32 96 Preps
Product Code 13565230
Thermo Scientific™ Elution Buffer for MagJET Plasmid DNA Kit
Product Code 16336542
Thermo Scientific™ KingFisher™ Duo-Ready DNA Ultra2.0 Prefilled Plates
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Product Code 4926479
Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit
Product Code 4926452
Invitrogen™ UltraPure™ TAE Buffer, 10X
Product Code 10202540
Thermo Scientific™ Silica Bead DNA Gel Extraction Kit
Product Code 10571031
Thermo Scientific™ Genomic DNA Purification Kit
Product Code 3973089
Cytiva TempliPhi™ 100/500 Amplification Kit
Product Code 15341672
Thermo Scientific™ MagJET Separation Rack, 96-well plate
Product Code 4520372
Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit
Product Code 4926484
Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit
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Product Code 7161465
Implen™ NanoPhotometer™ N60 Micro-Volume UV-VIS Spectrophotometer
Product Code 4528904
Thermo Scientific™ Owl™ EasyCast™ B2 Mini Gel Electrophoresis Systems
FAQ
The purpose of DNA extraction and purification is to isolate DNA from cells or tissues so that it can be used for various downstream applications. These applications include:
- Genetic Research: Studying the structure, function, and regulation of genes
- Diagnostics: Identifying genetic disorders, infectious diseases, and other health conditions
- Forensic Analysis: Analyzing DNA from crime scenes for identification purposes
- Biotechnology: Genetic engineering, cloning, and production of genetically modified organisms (GMOs)
- Pharmaceutical Development: Discovering and developing new drugs and therapies
- Agricultural Science: Breeding plants and animals with desirable traits
DNA extraction and purification ensure that the DNA obtained is free from contaminants such as proteins, lipids, and other cellular components that could interfere with these applications.
To purify DNA after extraction, common methods include:
- Phenol-Chloroform Extraction: Removes proteins using phenol and chloroform, followed by ethanol precipitation.
- Ethanol Precipitation: DNA is precipitated with ethanol and sodium acetate, then washed with 70% ethanol.
- Silica Column-Based Methods: DNA binds to silica columns, contaminants are washed away, and DNA is eluted.
- Magnetic Bead-Based Methods: DNA binds to magnetic beads, contaminants are washed away, and DNA is eluted.
- Cesium Chloride Density Gradient Centrifugation: Uses ultracentrifugation to separate DNA based on density.
Each method is chosen based on the purity requirements and resources available.
Filtering is important in DNA extraction for several reasons:
- Removal of Debris: It helps to eliminate particulate matter, such as cell fragments and undissolved materials, that could interfere with subsequent steps in the extraction process
- Improving Purity: By removing contaminants, filtering ensures that the extracted DNA is cleaner and more suitable for downstream applications like PCR, sequencing, and cloning
- Preventing Equipment Damage: Filtering protects laboratory equipment (e.g., centrifuges, pipettes) from clogging and potential damage caused by debris
- Enhancing Accuracy: Cleaner samples lead to more accurate and reliable results in genetic analysis and other applications
Overall, filtering is a crucial step to ensure the quality and integrity of the extracted DNA.
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