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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Phase

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Technique

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1 – 15 of 852 results
1
Promotions Available

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
2
Promotions Available

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3

Thermo Scientific™ Hypersil GOLD™ PEI HILIC HPLC Columns

Retain highly polar and hydrophilic analytes, which are difficult to analyze with conventional C18 columns, using Hypersil GOLD PEI HILIC LC Columns.

Pore Size 175 Å
Carbon Load 6%
Stationary Phase Polyethyleneimine
Temperature 60°C
Phase HILIC
Endcapped No
Column Type HILIC
pH 2 to 8
Surface Area 220 m²/g
4
Promotions Available

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
5
Promotions Available

Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns

Use as a robust, general-purpose column for QC/QA applications. They are high-quality, base-deactivated, fully endcapped, have similar C18 selectivity but slightly less retentive.

EDGE
Pore Size 130 Å
Carbon Load 7%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L7
6

Thermo Scientific™ EASY-Spray™ HPLC Columns
BIOPHARMA

Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.

EDGE
Phase Reversed Phase
7

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
BIOPHARMA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
8

Thermo Scientific™ NativePac OBE-1 SEC Column for Online Sample Preparation

Perform Native-MS analysis of biomacromolecules with the NativePac OBE-1 SEC Column designed for rapid exchange of non-volatile buffer components with a volatile buffer.

EDGE
9

Thermo Scientific™ Single nanoViper™ and Double nanoViper™ PepMap™ Neo UHPLC Columns
BIOPHARMA

Separate challenging peptide mapping samples with Thermo Scientific™ Double nanoViper™ PepMap™ Neo UHPLC columns.

EDGE
Pore Size 100 Å
Particle Size 2 μm
Carbon Load 15%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 8
For Use With (Application) Peptide Mapping, LC-MS
Max. Pressure 22,000 psi (1500 bar)
USP Type L1
10

Thermo Scientific™ POROS™ GoPure™ XS Pre-packed Column, 0.8 x 10 cm, 5 mL

Thermo Scientific™ POROS™ GoPure™ 0.8 cm pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification.

EDGE
11
Promotions Available

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
BIOPHARMA

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

EDGE
12

Thermo Scientific™ ProPac™ 3R SAX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SAX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and AAVs.

EDGE
Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Proprietary
Temperature Ambient to 60°C
Phase Strong Anion Exchange
Packing Material Monodisperse Polymer Resin
Column Type Ion Exchange
pH 2 to 12
Technique Bio-LC
Max. Pressure 4500 psi (310 bar)
Product Line ProPac
Column Format Analytical Column
13

Thermo Scientific™ Acclaim™ Surfactant Plus HPLC Columns

Use for simultaneous separation of anionic, nonionic, cationic, and amphoteric surfactants with CAD and LC-MS detection.

EDGE
Pore Size 120 Å
Stationary Phase Proprietary
Temperature 50°C
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Specialty/Application Specific
pH 2.5 to 7.5
Green Features Responsibly packaged
Surface Area 300 m²/g
Product Line Acclaim
14

Thermo Scientific™ µPAC™ Neo HPLC Columns

Learn more about your proteome samples with Thermo Scientific™ μPAC™ Neo Plus HPLC columns.

EDGE
Pore Size 100 to 300 Å
Stationary Phase C18
Endcapped Yes
Packing Material Silicon Chip
pH 1.5 to 7
Max. Pressure 6526 psi (450 bar)
15

Thermo Scientific™ Acclaim™ Trinity P2 HPLC Columns

Use these mixed-mode columns for separation of multivalent cations or anions and pharmaceuticals.

EDGE
Pore Size 300 Å
Solvents Compatible with most common HPLC organic solvents except for alcohols
Stationary Phase Spherical, Nanopolymer Hybrid Silica
Temperature 45°C
Phase HILIC and Ion Exchange
Endcapped Proprietary
Column Type Mixed Mode
Green Features Responsibly packaged
Surface Area 100 m²/g
Max. Pressure 6000 psi (415 bar)
Particle Size 3 μm
Mobile Phase Compatibility 0 to 100% aqueous mobile phase
pH 2.5 to 7
Particle Shape Spherical silica
Product Line Acclaim