Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Cytiva MBPTrap™ HP Prepacked Columns
Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.
| Buffer | Aqueous Buffer |
|---|---|
| Height (Metric) | 25mm |
| Content And Storage | 2° to 8°C |
| Bed Volume | 1mL |
| pH Range | >7 |
| Column Type | Ion Exchange Column |
| Material | Polypropylene |
| For Use With (Application) | Purification of MBP tagged Proteins |
| Max. Pressure | 3 bar |
| Particle Size | 34μm |
| Ligand Type | Dextrin |
| Product Type | Dextrin Sepharose HP prepacked column |
| Support Type | 6% Highly Cross-linked Agarose |
| Height (English) | 0.98 in. |
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 2 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva StrepTrap XT Chromatography Column
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
| Height (Metric) | 25 mm |
|---|---|
| Column Type | Affinity Column |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva Protein G HP SpinTrap™ Columns
For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva Tricorn™ Filter Kit
Used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Cytiva Tricorn™ Filter Kit includes top and bottom adapters, bottom filters, and EPDM O-rings, five of each
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 45 to 165 μm |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Cytiva™ Whatman™ Qualitative Filter Paper Grade CF 12
Whatman™ Grade CF 12 qualitative filter paper is ideal for general laboratory applications, such as removing particulate matter, clarifying liquids and separating solids from liquids in qualitative analysis. The Grade CF 12 offers medium retention and flow rate.
Cytiva Superose™ 6 Increase Columns
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
| Particle Size | 8.6μm |
|---|---|
| Ligand Type | Cross-linked agarose |
| Content And Storage | 4° to 30° in 20% ethanol |
| pH Range | 3 to 12 |
| Column Type | Gel Filtration Column |
| Material | Borosilicate glass |
| Max. Pressure | 435 psi |
Cytiva Tricorn Columns
Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.
| Includes | Glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock and filter holder |
|---|---|
| Product Type | Tricorn Columns (Empty) |
| Quantity | 1 Ea. |
| Material | Borosilicate glass |
| For Use With (Application) | High resolution liquid chromatography of biomolecules performed at medium to high pressures |
| Support Type | Polypropylene filter |