The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
Store the polymer pouch at 2-8°C until ready to use. Note that expired pouches cannot be used on the instrument.
On-instrument life/usage
3500 Dx (8 capillary) Genetic Analyzer: lesser of 7 days or 384 samples or 60 injections
3500xL Dx (24 capillary) Genetic Analyzer: lesser of 7 days or 384 samples or 20 injections
The pouch includes additional polymer volume to accommodate both installation and a reasonable number of post-installation wizard operations such as bubble removal without affecting the number of samples/injections available.
Capillary Length
50 cm, 36 cm
Test Time
2 hr. for 50 cm capillary (long read), 1 hr. for 36 cm capillary (std. seq.)
The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
The Invitrogen Collibri ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.