accessibility menu, dialog, popup

UserName

Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (52)
Modifying Enzymes (10)
Restriction Enzyme Buffers (4)

Filtered Search Results

Products from some of our suppliers do not display in filtered search results. Please clear all filters to see these products.

Narrow Results

Narrow Results

category

special offers

  • (2)

brands

  • (64)
  • (2)

Concentration

  • (2)
  • (1)
  • (47)
  • (2)
  • (1)
  • (1)
  • (2)
Show all

Form

  • (58)
  • (1)
Keyword Search:
1 – 15 of 58 results
1

OPTIZYME™ SmaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site CCC.GGG
Form Liquid
Product Type SmaI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 30°C
Concentration 10 U/μL
For Use With (Application) >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI
2

OPTIZYME™ XbaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site T.CTAGA
Form Liquid
Product Type XbaI
Storage Buffer 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
For Use With (Application) Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme
3

OPTIZYME™ NotI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site GC.GGCCGC
Content And Storage Keep container tightly closed
Form Liquid
Product Type NotI
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Concentration 10 U/μL
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
4

OPTIZYME™ BglII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site A.GATCT
Content And Storage Keep container tightly closed
Form Liquid
Product Type BglII
Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
5

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Components >50% Water, 25 to 50% Glycerin
Cut Site AT.CGAT
Content And Storage Keep container tightly closed
Form Liquid
Product Type ClaI (Bsu15I)
Storage Buffer 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
9

OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type T4 DNA Polymerase
Storage Buffer OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Color Colorless
pH 7.5
Concentration 5 U/μL
For Use With (Application) Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Source E. coli cells with a cloned gene 43 of bacteriophage T4
10

OPTIZYME™ DNA Polymerase I, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type DNA Polymerase I
Storage Buffer OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM
Color Colorless
pH 7.5
Concentration 10 U/μL
For Use With (Application) DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases
Source E. coli cells with a cloned polA gene
14
Promotions Available

Thermo Scientific™ KpnI Buffer (10X)

Optimize digestions with KpnI restriction enzyme using this buffer, premixed with BSA for enhanced stability.

15
Promotions Available

Thermo Scientific™ AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X)

Optimize digestions with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes using this buffer.