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Cytiva HiTrap™ Protein G HP Prepacked Columns
Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.
| Content And Storage | 4°C to 30°C |
|---|---|
| Description | HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat. |
| Storage Buffer | 20% Ethanol |
| Product Type | Pre-packed Columns |
| For Use With (Application) | Designed for use with a syringe, peristaltic pump, or chromatography system |
Cytiva HiTrap™ MabSelect™ SuRe Prepacked Columns
Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Binding Capacity | ≈35mg Human lgG/mL at 2.4 min. residence time |
| For Use With (Application) | Chromatography System, Manual |
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap Q FF |
Cytiva HiTrap™ IEX HP Prepacked Columns
Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10mg Heparin/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 5 to 10 |
| Product Type | Heparin HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HiTrap™ DEAE Sepharose Fast Flow IEX Columns
Weak anion exchanger for small-scale protein purifications. Cytiva Lifescience™ HiTrap™ DEAE Sepharose Fast Flow IEX Columns are convenient and rapid.
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap DEAE FF |
| For Use With (Application) | For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules |
Cytiva HiTrap™ Q Sepharose XL IEX Columns
Optimized for fast, convenient small-scale protein capture
| Content And Storage | 4°C to 30°C in 20% Ethanol |
|---|---|
| Description | HiTrap Q XL |
| For Use With (Application) | HiTrap Q XL are strong anion exchangers prepacked with Q Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography. |
Cytiva HiTrap™ Streptavidin HP Column
Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.
Cytiva HiTrap™ Protein A HP Prepacked Columns
Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.
| Content And Storage | 4°C to 8°C, 20% Ethanol |
|---|---|
| For Use With (Equipment) | For syringe, peristaltic pump or a chromatography system |
| Binding Capacity | 20 mg/mL IgG Medium |
| For Use With (Application) | Chromatography System, Manual |
Cytiva HiTrap™ Blue HP Columns
For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins
| Particle Size | 34 μm |
|---|---|
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 4 to 12 |
| Product Type | Blue HP Columns |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Sample Type | Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins |
| Material | Polypropylene |
| For Use With (Application) | Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva HiTrap Capto S ImpAct Prepacked Columns
HiTrap Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).
| Particle Size | 50μm |
|---|---|
| pH Range | 4 to 12 |
| Product Type | HiTrap Capto S ImpAct Prepacked Column |
| Column Type | Ion Exchange Column |
| Material | High-flow agarose |
Cytiva HiTrap™ SP Sepharose XL IEX Columns
Optimized for fast, convenient small-scale protein capture
| Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
|---|---|
| Description | HiTrap SP XL |
| For Use With (Application) | HiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography. |
Cytiva HiTrap™ IMAC Columns
For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion
| Flow Rate | <4 mL/min. |
|---|---|
| Content And Storage | 4°C to 30°C |
| pH Range | 3 to 12 |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system |
| Material | Polypropylene |
| For Use With (Application) | For optimizing purification of His-tagged Proteins |
| Max. Pressure | 42 psi |
| Particle Size | 45 to 165 μm |
|---|---|
| Ligand Type | Butyl |
| Height (Metric) | 25 mm |
| pH Range | 3 to 13 |
| Product Type | HiTrap Butyl FF |
| Material | Polypropylene |
| Storage Requirements | 4°C to 30°C, 20% Ethanol |
| For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
| Support Type | 4% Cross-linked Agarose |
| Media Type | Butyl Sepharose 4 Fast Flow |
| Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
| Height (English) | 0.984 in. |